論文 - 詳細
| RRC ID | 33468 |
|---|---|
| 著者 | Chang AC, Cohen SN. |
| タイトル | Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. |
| ジャーナル | J Bacteriol |
| Abstract |
Construction and characterization of a class of multicopy plasmid cloning vehicles containing the replication system of miniplasmid P15A are described. The constructed plasmids have cleavage sites within antibiotic resistance genes for a variety of commonly employed site-specific endonucleases, permitting convenient use of the insertional inactivation procedure for the selection of clones that contain hybrid DNA molecules. Although the constructed plasmids showed DNA sequence homology with the ColE1 plasmid within the replication region, were amplifiable by chloramphenicol or spectinomycin, required DNA polymerase I for replication, and shared other replication properties with ColE1, they were nevertheless compatible with ColE1. P15A-derived plasmids were not self-transmissible and were mobilized poorly by Hfr strains; however, mobilization was complemented by the presence of a ColE1 plasmid within the same cell. |
| 巻・号 | 134(3) |
| ページ | 1141-56 |
| 公開日 | 1978-6-1 |
| DOI | 10.1128/jb.134.3.1141-1156.1978 |
| PMID | 149110 |
| PMC | PMC222365 |
| MeSH | Chloramphenicol / pharmacology Chromosome Mapping DNA Restriction Enzymes / metabolism DNA, Bacterial / analysis DNA, Bacterial / genetics* DNA, Recombinant / genetics* Escherichia coli / analysis Escherichia coli / genetics* Nucleic Acid Conformation Plasmids* / drug effects Spectinomycin / pharmacology Transformation, Bacterial |
| IF | 3.006 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 196 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| 原核生物(大腸菌) | pACYC177 pACYC184 |