RRC ID 33471
Author Cheng SC, Modrich P.
Title Positive-selection cloning vehicle useful for overproduction of hybrid proteins.
Journal J Bacteriol
Abstract Plasmid pSCC31 contains the EcoRI endonuclease gene downstream from lambda pL. It does not yield transformants upon introduction into Escherichia coli unless the structural integrity of the endonuclease is destroyed. This makes it useful as a positive-selection cloning vehicle which can be employed for regulated overproduction of hybrid proteins.
Volume 154(2)
Pages 1005-8
Published 1983-5-1
DOI 10.1128/jb.154.2.1005-1008.1983
PMID 6302074
PMC PMC217560
MeSH Cloning, Molecular* Coliphages / genetics DNA Restriction Enzymes / genetics* Deoxyribonuclease EcoRI Escherichia coli / genetics Genes Genes, Viral Genetic Vectors Plasmids* Protein Biosynthesis* Viral Proteins / biosynthesis
IF 3.006
Times Cited 46
Prokaryotes E. coli pSCC31-B12