RRC ID 33472
Author Yasuda S, Takagi T.
Title Overproduction of Escherichia coli replication proteins by the use of runaway-replication plasmids.
Journal J Bacteriol
Abstract A derivative of the runaway-replication plasmid was constructed. This plasmid, pSY343, has the gene for kanamycin resistance and single sites for EcoRI, BamHI, HindIII, KpnI, and XhoI that can be used as cloning sites without inactivating the kanamycin resistance gene or the replication genes. Three replication genes of Escherichia coli were cloned on the plasmid. The activity of dnaA, dnaZ, and ssb gene products were 200-, 90-, and 60-fold greater, respectively, in the cells containing these plasmids than in normal cells.
Volume 154(3)
Pages 1153-61
Published 1983-6-1
DOI 10.1128/jb.154.3.1153-1161.1983
PMID 6304005
PMC PMC217586
MeSH Bacterial Proteins / biosynthesis Bacterial Proteins / genetics* Carrier Proteins / biosynthesis Carrier Proteins / genetics* Cloning, Molecular DNA Replication* Escherichia coli / genetics* Escherichia coli / metabolism Genes, Bacterial Genetic Vectors Plasmids*
IF 3.006
Times Cited 49
Prokaryotes E. coli pSY343