RRC ID 33497
著者 Vidal-Ingigliardi D, Raibaud O.
タイトル A convenient technique to compare the efficiency of promoters in Escherichia coli.
ジャーナル Nucleic Acids Res
Abstract We describe a technique which allows one to insert any promoter in front of the chromosomal malPQ operon. This can be done easily by using only one plasmid, one strain, and two simple selections. Properties of the final chromosomal fusion are such that the level of amylomaltase, the product of the malQ gene, measures quantitatively the efficiency of the inserted promoter. This method was utilized to compare the efficiency of four well-known promoters: lacZp, trp, tac, lambdaPR and three malT activated promoters: malPp, malkP and malEp.
巻・号 13(16)
ページ 5919-26
公開日 1985-8-26
DOI 10.1093/nar/13.16.5919
PMID 3162159
PMC PMC321922
MeSH Chromosomes, Bacterial Escherichia coli / genetics* Genotype Glucosyltransferases / genetics Glycogen Debranching Enzyme System* Operon* Plasmids Species Specificity Transcription, Genetic
IF 11.502
引用数 62
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(大腸菌) pOM41