論文 - 詳細
| RRC ID | 33510 |
|---|---|
| 著者 | Sage DR, Chillemi AC, Fingeroth JD. |
| タイトル | A versatile prokaryotic cloning vector with six dual restriction enzyme sites in the polylinker facilitates efficient subcloning into vectors with unique cloning sites. |
| ジャーナル | Plasmid |
| Abstract |
In large and complex vectors a single restriction enzyme recognition site may be available for introduction of additional DNA requiring the development of linker fragments to create compatible insertion sites. This technology can be time consuming and costly. We describe the construction of a simple phagemid, pSFI, with a polylinker that contains six pairs of dual, rare-cutting, restriction enzyme recognition sites (NotI, SpeI, EcoRV, PstI, SacII, EagI) with multiple unique sites between each pair. This has permitted rapid subcloning of DNA with creation of single flanking restriction enzyme sites. pSFI was used to expedite transfer of viral genes to a LacZ-inducible expression vector and to an adenovirus expression cassette for production of replication-defective virus. The use of this phagemid has facilitated complex vector manipulations and is a valuable adjunct to the family of multifunctional cloning vectors. |
| 巻・号 | 40(2) |
| ページ | 164-8 |
| 公開日 | 1998-9-1 |
| DOI | 10.1006/plas.1998.1357 |
| PII | S0147-619X(98)91357-9 |
| PMID | 9735318 |
| MeSH | Bacteriophages / genetics* Base Sequence Binding Sites DNA Restriction Enzymes / metabolism DNA, Viral Genetic Vectors* Molecular Sequence Data Prokaryotic Cells |
| IF | 2.805 |
| 引用数 | 3 |
| WOS 分野 | GENETICS & HEREDITY MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | pSF1 |