RRC ID 3353
Author Iwata K, Shindome C, Kobayashi Y, Takeda M, Yamashita O, Shiomi K, Fujiwara Y.
Title Temperature-dependent activation of ERK/MAPK in yolk cells and its role in embryonic diapause termination in the silkworm Bombyx mori.
Journal J. Insect Physiol.
Abstract The silkworm Bombyx mori requires 2-3 months of low temperature (5 degrees C) to terminate embryonic diapause. The molecular mechanisms, however, are unknown. Extracellular signal-regulated kinase (ERK)/mitogen-activated protein kinase (MAPK) is temperature-dependently activated in the yolk cells of diapausing eggs after 45 days at 5 degrees C, coincident with the acquisition of developmental competence of the embryos at 25 degrees C. Yolk cell granulation and dissociation also begin in diapause eggs incubated at 5 degrees C for 45 days. We used dechorionated egg culture as a model system of diapause termination and observed that both yolk cell dissociation and embryonic development are inhibited by MAPK-ERK kinase (MEK) inhibitor U0126. Therefore, we suggest that ERK in yolk cells has a role in regulating changes in yolk morphology and termination of embryonic diapause in B. mori.
Volume 51(12)
Pages 1306-12
Published 2005-12
DOI 10.1016/j.jinsphys.2005.07.009
PII S0022-1910(05)00164-2
PMID 16169554
MeSH Adaptation, Physiological / physiology* Animals Blotting, Western Bombyx / drug effects Bombyx / embryology* Bombyx / enzymology Butadienes / toxicity Enzyme Activation / physiology Extracellular Signal-Regulated MAP Kinases / antagonists & inhibitors Extracellular Signal-Regulated MAP Kinases / metabolism* Immunohistochemistry Nitriles / toxicity Ovum / cytology Ovum / drug effects Ovum / metabolism* Phosphorylation / drug effects Temperature*
IF 2.733
Times Cited 27
Silkworms ?