| RRC ID |
3353
|
| 著者 |
Iwata K, Shindome C, Kobayashi Y, Takeda M, Yamashita O, Shiomi K, Fujiwara Y.
|
| タイトル |
Temperature-dependent activation of ERK/MAPK in yolk cells and its role in embryonic diapause termination in the silkworm Bombyx mori.
|
| ジャーナル |
J Insect Physiol
|
| Abstract |
The silkworm Bombyx mori requires 2-3 months of low temperature (5 degrees C) to terminate embryonic diapause. The molecular mechanisms, however, are unknown. Extracellular signal-regulated kinase (ERK)/mitogen-activated protein kinase (MAPK) is temperature-dependently activated in the yolk cells of diapausing eggs after 45 days at 5 degrees C, coincident with the acquisition of developmental competence of the embryos at 25 degrees C. Yolk cell granulation and dissociation also begin in diapause eggs incubated at 5 degrees C for 45 days. We used dechorionated egg culture as a model system of diapause termination and observed that both yolk cell dissociation and embryonic development are inhibited by MAPK-ERK kinase (MEK) inhibitor U0126. Therefore, we suggest that ERK in yolk cells has a role in regulating changes in yolk morphology and termination of embryonic diapause in B. mori.
|
| 巻・号 |
51(12)
|
| ページ |
1306-12
|
| 公開日 |
2005-12-1
|
| DOI |
10.1016/j.jinsphys.2005.07.009
|
| PII |
S0022-1910(05)00164-2
|
| PMID |
16169554
|
| MeSH |
Adaptation, Physiological / physiology*
Animals
Blotting, Western
Bombyx / drug effects
Bombyx / embryology*
Bombyx / enzymology
Butadienes / toxicity
Enzyme Activation / physiology
Extracellular Signal-Regulated MAP Kinases / antagonists & inhibitors
Extracellular Signal-Regulated MAP Kinases / metabolism*
Immunohistochemistry
Nitriles / toxicity
Ovum / cytology
Ovum / drug effects
Ovum / metabolism*
Phosphorylation / drug effects
Temperature*
|
| IF |
2.246
|
| 引用数 |
34
|
|
WOS 分野
|
ENTOMOLOGY
ZOOLOGY
PHYSIOLOGY
|
| リソース情報 |
| カイコ |
|
