| RRC ID |
33532
|
| Author |
Yan J, Aboshi T, Teraishi M, Strickler SR, Spindel JE, Tung CW, Takata R, Matsumoto F, Maesaka Y, McCouch SR, Okumoto Y, Mori N, Jander G.
|
| Title |
The Tyrosine Aminomutase TAM1 Is Required for β-Tyrosine Biosynthesis in Rice.
|
| Journal |
Plant Cell
|
| Abstract |
Non-protein amino acids, often isomers of the standard 20 protein amino acids, have defense-related functions in many plant species. A targeted search for jasmonate-induced metabolites in cultivated rice (Oryza sativa) identified (R)-β-tyrosine, an isomer of the common amino acid (S)-α-tyrosine in the seeds, leaves, roots, and root exudates of the Nipponbare cultivar. Assays with 119 diverse cultivars showed a distinct presence/absence polymorphism, with β-tyrosine being most prevalent in temperate japonica cultivars. Genetic mapping identified a candidate gene on chromosome 12, which was confirmed to encode a tyrosine aminomutase (TAM1) by transient expression in Nicotiana benthamiana and in vitro enzyme assays. A point mutation in TAM1 eliminated β-tyrosine production in Nipponbare. Rice cultivars that do not produce β-tyrosine have a chromosome 12 deletion that encompasses TAM1. Although β-tyrosine accumulation was induced by the plant defense signaling molecule jasmonic acid, bioassays with hemipteran and lepidopteran herbivores showed no negative effects at physiologically relevant β-tyrosine concentrations. In contrast, root growth of Arabidopsis thaliana and other tested dicot plants was inhibited by concentrations as low as 1 μM. As β-tyrosine is exuded into hydroponic medium at higher concentrations, it may contribute to the allelopathic potential of rice.
|
| Volume |
27(4)
|
| Pages |
1265-78
|
| Published |
2015-4-1
|
| DOI |
10.1105/tpc.15.00058
|
| PII |
tpc.15.00058
|
| PMID |
25901084
|
| PMC |
PMC4558700
|
| MeSH |
Gene Expression Regulation, Plant
Oryza / enzymology*
Oryza / metabolism*
Plant Proteins / genetics
Plant Proteins / metabolism*
Tyrosine / biosynthesis*
|
| IF |
9.618
|
| Times Cited |
19
|
|
WOS Category
|
PLANT SCIENCES
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| Rice |
Induced Mutation Lines |
