論文 - 詳細
| RRC ID | 33544 |
|---|---|
| 著者 | Serizawa M, Yamamoto H, Yamaguchi H, Fujita Y, Kobayashi K, Ogasawara N, Sekiguchi J. |
| タイトル | Systematic analysis of SigD-regulated genes in Bacillus subtilis by DNA microarray and Northern blotting analyses. |
| ジャーナル | Gene |
| Abstract |
The SigD-regulated genes in Bacillus subtilis were systematically analyzed by comparing the pattern of transcripts derived from wild-type cells with those from sigD mutant cells using DNA microarray technology. One hundred and fifty-eight genes were found to be SigD-dependent candidates, 46 of which being known SigD-regulated genes. Northern blot analysis revealed that 18 of the remaining genes were SigD-dependent. The SigD consensus sequence was newly identified in the upstream regions of nine operons (11 genes): ybdO, yfmT-yfmS, hemAT, yjcP-yjcQ, yjfB, ylqB, yoaH, yscB and yxkC, and the other seven genes were assumed to be indirectly affected by a SigD mutation. Furthermore, yviE-yviF are likely to be SigD-dependent genes, because three independent sets of array data for yviE and yviF indicated they are SigD-dependent, and these genes are neighbors of flgL and hag transcribed by SigD RNA polymerase. |
| 巻・号 | 329 |
| ページ | 125-36 |
| 公開日 | 2004-3-31 |
| DOI | 10.1016/j.gene.2003.12.024 |
| PII | S0378111903011661 |
| PMID | 15033535 |
| MeSH | Bacillus subtilis / genetics* Base Sequence Blotting, Northern DNA, Complementary / genetics Gene Expression Regulation, Bacterial* Genes, Bacterial / genetics* Oligonucleotide Array Sequence Analysis RNA, Messenger / genetics RNA, Messenger / metabolism Regulon / genetics Sigma Factor / metabolism* Transcription, Genetic |
| IF | 2.984 |
| 引用数 | 56 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 原核生物(枯草菌) | MBS61 |