| RRC ID |
33546
|
| Author |
Murayama R, Akanuma G, Makino Y, Nanamiya H, Kawamura F.
|
| Title |
Spontaneous transformation and its use for genetic mapping in Bacillus subtilis.
|
| Journal |
Biosci Biotechnol Biochem
|
| Abstract |
Using a simple semi-synthetic competence and sporulation medium (CSM), we found evidence that Bacillus subtilis cells transformed in the competence phase can sporulate, indicating that genetic information acquired during the competence phase is inherited by the next generation after germination of the transformed spores. Moreover, the results from mixed cell culture experiments suggest that spontaneous genetic transformation can occur between competent cells and DNA released from lysed cells in the natural environment. We also found evidence that the spontaneous transformation system can be used for genetic mapping in B. subtilis.
|
| Volume |
68(8)
|
| Pages |
1672-80
|
| Published |
2004-8-1
|
| DOI |
10.1271/bbb.68.1672
|
| PII |
JST.JSTAGE/bbb/68.1672
|
| PMID |
15322350
|
| MeSH |
Bacillus subtilis / genetics*
Bacillus subtilis / growth & development
Bacillus subtilis / physiology
Chromosome Mapping*
DNA, Bacterial / genetics*
Spores / physiology*
Transformation, Genetic*
|
| IF |
1.516
|
| Times Cited |
11
|
|
WOS Category
|
CHEMISTRY, APPLIED
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Prokaryotes B. subtilis |
MBS419 |
