RRC ID 33549
著者 Tojo S, Satomura T, Morisaki K, Deutscher J, Hirooka K, Fujita Y.
タイトル Elaborate transcription regulation of the Bacillus subtilis ilv-leu operon involved in the biosynthesis of branched-chain amino acids through global regulators of CcpA, CodY and TnrA.
ジャーナル Mol Microbiol
Abstract The Bacillus subtilis ilv-leu operon involved in the biosynthesis of branched-chain amino acids is under negative regulation mediated by TnrA and CodY, which recognize and bind to their respective cis-elements located upstream of the ilv-leu promoter. This operon is known to be under CcpA-dependent positive regulation. We have currently identified a catabolite-responsive element (cre) for this positive regulation (bases -96 to -82; +1 is the ilv-leu transcription initiation base) by means of DNase I-footprinting in vitro, and deletion and base-substitution analyses of cre. Under nitrogen-rich growth conditions in glucose-minimal medium supplemented with glutamine and amino acids, CcpA and CodY exerted positive and negative regulation of ilv-leu, respectively, but TnrA did not function. Moreover, CcpA and CodY were able to function without their counteracting regulation of each other, although the CcpA-dependent positive regulation did not overcome the CodY-dependent negative regulation. Furthermore, under nitrogen-limited conditions in glucose-minimal medium with glutamate as the sole nitrogen source, CcpA and TnrA exerted positive and negative regulation, respectively, but CodY did not function. This CcpA-dependent positive regulation occurred without the TnrA-dependent negative regulation. However, the TnrA-dependent negative regulation did not occur without the CcpA-dependent positive regulation, raising the possibility that this negative regulation might decrease the CcpA-dependent positive regulation. The physiological role of this elaborate transcription regulation of the B. subtilis ilv-leu operon in overall metabolic regulation in this organism is discussed.
巻・号 56(6)
ページ 1560-73
公開日 2005-6-1
DOI 10.1111/j.1365-2958.2005.04635.x
PII MMI4635
PMID 15916606
MeSH Amino Acids, Branched-Chain / biosynthesis* Bacillus subtilis / genetics Bacillus subtilis / growth & development Bacillus subtilis / metabolism* Bacterial Proteins / genetics Bacterial Proteins / metabolism* Base Sequence DNA Footprinting DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Gene Expression Regulation, Bacterial* Isoleucine / biosynthesis Leucine / biosynthesis Molecular Sequence Data Nitrogen / metabolism Operon* Repressor Proteins / genetics Repressor Proteins / metabolism Response Elements / genetics Transcription Factors / genetics Transcription Factors / metabolism Transcription, Genetic* Valine / biosynthesis
IF 3.418
引用数 75
WOS 分野 MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(枯草菌) MBS263 MBS267 MBS269 MBS272 MBS273 MBS275 MBS276 MBS277 MBS278 MBS279