Reference - Detail
| RRC ID | 33561 |
|---|---|
| Author | Yamamoto H, Hashimoto M, Higashitsuji Y, Harada H, Hariyama N, Takahashi L, Iwashita T, Ooiwa S, Sekiguchi J. |
| Title | Post-translational control of vegetative cell separation enzymes through a direct interaction with specific inhibitor IseA in Bacillus subtilis. |
| Journal | Mol Microbiol |
| Abstract |
Three D,L-endopeptidases, LytE, LytF and CwlS, are involved in the vegetative cell separation in Bacillus subtilis. A novel cell surface protein, IseA, inhibits the cell wall lytic activities of these d,l-endopeptidases in vitro, and IseA negatively regulates the cell separation enzymes at the post-translational level. Immunofluorescence microscopy indicated that the IseA-3xFLAG fusion protein was specifically localized at cell separation sites and poles on the vegetative cell surface in a similar manner of the d,l-endopeptidases. Furthermore, pull-down assay showed that IseA binds to the catalytic domain of LytF, indicating that IseA is localized on the cell surface through the catalytic domain of LytF. Overexpression of IseA caused a long-chained cell morphology in the exponential growth phase, indicating that IseA inhibits the cell separation D,L-endopeptidases in vivo. Besides, overexpression of IseA in a cwlO disruptant affected cell growth, implying that IseA is also involved in the cell elongation event. However, although IseA inhibits the activities of LytE, LytF, CwlS and CwlO in vitro, it is unlikely to inhibit CwlS and CwlO in vivo. This is the first demonstration that the cell separation event is post-translationally controlled through a direct interaction between cell separation enzymes and a specific novel inhibitor in bacteria. |
| Volume | 70(1) |
| Pages | 168-82 |
| Published | 2008-10-1 |
| DOI | 10.1111/j.1365-2958.2008.06398.x |
| PII | MMI6398 |
| PMID | 18761694 |
| MeSH | Bacillus subtilis / enzymology* Bacillus subtilis / genetics Bacillus subtilis / growth & development Bacterial Proteins / metabolism* Carrier Proteins / metabolism* Catalytic Domain Cell Wall / enzymology* DNA, Bacterial / genetics Endopeptidases / metabolism* Enzyme Inhibitors / metabolism Genes, Bacterial Membrane Proteins / metabolism Microscopy, Fluorescence Plasmids Protein Processing, Post-Translational* |
| IF | 3.418 |
| Times Cited | 24 |
| WOS Category | MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes B. subtilis | MBS39 MBS40 MBS41 MBS42 MBS43 MBS44 MBS45 MBS46 MBS47 |