RRC ID 33572
著者 Kobayashi K, Sudiarta IP, Kodama T, Fukushima T, Ara K, Ozaki K, Sekiguchi J.
タイトル Identification and characterization of a novel polysaccharide deacetylase C (PdaC) from Bacillus subtilis.
ジャーナル J Biol Chem
Abstract Cell wall metabolism and cell wall modification are very important processes that bacteria use to adjust to various environmental conditions. One of the main modifications is deacetylation of peptidoglycan. The polysaccharide deacetylase homologue, Bacillus subtilis YjeA (renamed PdaC), was characterized and found to be a unique deacetylase. The pdaC deletion mutant was sensitive to lysozyme treatment, indicating that PdaC acts as a deacetylase. The purified recombinant and truncated PdaC from Escherichia coli deacetylated B. subtilis peptidoglycan and its polymer, (-GlcNAc-MurNAc[-L-Ala-D-Glu]-)(n). Surprisingly, RP-HPLC and ESI-MS/MS analyses showed that the enzyme deacetylates N-acetylmuramic acid (MurNAc) not GlcNAc from the polymer. Contrary to Streptococcus pneumoniae PgdA, which shows high amino acid sequence similarity with PdaC and is a zinc-dependent GlcNAc deacetylase toward peptidoglycan, there was less dependence on zinc ion for deacetylation of peptidoglycan by PdaC than other metal ions (Mn(2+), Mg(2+), Ca(2+)). The kinetic values of the activity toward B. subtilis peptidoglycan were K(m) = 4.8 mM and k(cat) = 0.32 s(-1). PdaC also deacetylated N-acetylglucosamine (GlcNAc) oligomers with a K(m) = 12.3 mM and k(cat) = 0.24 s(-1) toward GlcNAc(4). Therefore, PdaC has GlcNAc deacetylase activity toward GlcNAc oligomers and MurNAc deacetylase activity toward B. subtilis peptidoglycan.
巻・号 287(13)
ページ 9765-9776
公開日 2012-3-23
DOI 10.1074/jbc.M111.329490
PII S0021-9258(20)65391-1
PMID 22277649
PMC PMC3323032
MeSH Bacillus subtilis / enzymology* Bacillus subtilis / genetics Bacterial Proteins / genetics Bacterial Proteins / metabolism* Escherichia coli / enzymology Escherichia coli / genetics Esterases / chemistry* Esterases / genetics Esterases / metabolism Gene Deletion Kinetics Peptidoglycan / chemistry* Peptidoglycan / genetics Peptidoglycan / metabolism Sequence Homology, Amino Acid Streptococcus pneumoniae / enzymology Streptococcus pneumoniae / genetics
IF 4.238
引用数 24
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(枯草菌) MBS35 MBS36 MBS37 MBS38