RRC ID 33585
著者 Fujita Y, Freese E.
タイトル Isolation and properties of a Bacillus subtilis mutant unable to produce fructose-bisphosphatase.
ジャーナル J Bacteriol
Abstract A Bacillus subtilis mutation (gene symbol fdpA1), producing a deficiency of D-fructose-1,6-bisphosphate 1-phosphohydrolase (EC 3.1.3.11, fructose-bisphosphatase), was isolated and genetically purified. An fdpA1-containing mutant did not produce cross-reacting material. It grew on any carbon source that allowed growth of the standard strain except myo-inositol and D-gluconate. Because the mutant could grow on D-fructose, glycerol, or L-malate as the sole carbon source, B. subtilis can produce fructose-6-phosphate and the derived cell wall precursors from these carbon sources in the absence of fructose-bisphosphatase. In other words, during gluconeogenesis B. subtilis must be able to bypass this reaction. Fructose-bisphosphatase is also not needed for the sporulation of B., subtilis. The fdpA1 mutation has the pleiotropic consequence that mutants carrying it cannot produce inositol dehydrogenase (EC 1.1.1.18) and gluconate kinase (EC 2.7.1.12) under conditions that normally induce these enzymes.
巻・号 145(2)
ページ 760-7
公開日 1981-2-1
DOI 10.1128/jb.145.2.760-767.1981
PMID 6257649
PMC PMC217176
MeSH Bacillus subtilis / enzymology Bacillus subtilis / genetics* Carbohydrate Metabolism Cross Reactions Fructose-Bisphosphatase / genetics* Fructose-Bisphosphatase / immunology Gluconates / biosynthesis Gluconates / metabolism Gluconeogenesis Inositol / biosynthesis Inositol / metabolism Phosphotransferases / biosynthesis Phosphotransferases (Alcohol Group Acceptor)* Spores, Bacterial / physiology Sugar Alcohol Dehydrogenases / biosynthesis
IF 3.006
引用数 58
WOS 分野 MICROBIOLOGY
リソース情報
原核生物(枯草菌) MBS68 MBS75 MBS84