RRC ID 33585
Author Fujita Y, Freese E.
Title Isolation and properties of a Bacillus subtilis mutant unable to produce fructose-bisphosphatase.
Journal J. Bacteriol.
Abstract A Bacillus subtilis mutation (gene symbol fdpA1), producing a deficiency of D-fructose-1,6-bisphosphate 1-phosphohydrolase (EC 3.1.3.11, fructose-bisphosphatase), was isolated and genetically purified. An fdpA1-containing mutant did not produce cross-reacting material. It grew on any carbon source that allowed growth of the standard strain except myo-inositol and D-gluconate. Because the mutant could grow on D-fructose, glycerol, or L-malate as the sole carbon source, B. subtilis can produce fructose-6-phosphate and the derived cell wall precursors from these carbon sources in the absence of fructose-bisphosphatase. In other words, during gluconeogenesis B. subtilis must be able to bypass this reaction. Fructose-bisphosphatase is also not needed for the sporulation of B., subtilis. The fdpA1 mutation has the pleiotropic consequence that mutants carrying it cannot produce inositol dehydrogenase (EC 1.1.1.18) and gluconate kinase (EC 2.7.1.12) under conditions that normally induce these enzymes.
Volume 145(2)
Pages 760-7
Published 1981-2
PMID 6257649
PMC PMC217176
MeSH Bacillus subtilis / enzymology Bacillus subtilis / genetics* Carbohydrate Metabolism Cross Reactions Fructose-Bisphosphatase / genetics* Fructose-Bisphosphatase / immunology Gluconates / biosynthesis Gluconates / metabolism Gluconeogenesis Inositol / biosynthesis Inositol / metabolism Phosphotransferases / biosynthesis Phosphotransferases (Alcohol Group Acceptor)* Spores, Bacterial / physiology Sugar Alcohol Dehydrogenases / biosynthesis
IF 3.234
Times Cited 58
WOS Category MICROBIOLOGY
Resource
Prokaryotes B. subtilis MBS68 MBS75 MBS84