RRC ID 33587
Author Nihashi J, Fujita Y.
Title Catabolite repression of inositol dehydrogenase and gluconate kinase syntheses in Bacillus subtilis.
Journal Biochim Biophys Acta
Abstract The regulation of induction of inositol dehydrogenase (EC and gluconate kinase (EC was studied in Bacillus subtilis. Inositol dehydrogenase is induced by myo-inositol and gluconate kinase is induced by D-gluconate. Both inductions were strongly repressed by rapidly metabolizable carbohydrates such as D-glucose, D-mannose, D-fructose and glycerol (D-glucose had the strongest repressive effect) but they were weakly repressed by slowly metabolizable carbohydrates. Although each carbohydrate exerted a stronger effect on the induction of inositol dehydrogenase than that of gluconate kinase, it showed a similar tendency with respect to the degree of repression of each induction. This catabolite repression could not be diminished by addition of cyclic AMP to medium. In addition, non-metabolizable D-glucose analogues had no or weak repressive effects. On the assumption that rapidly metabolizable carbohydrates might be metabolized to repress both inductions, it was investigated whether several mutants blocked in the Embden-Meyerhof pathway could produce metabolite(s) (repressor) to repress them. A phosphoglycerate kinase (EC deficient mutant could produce the repressor from D-glucose, D-mannose, D-fructose and glycerol but other mutants could not produce it from carbohydrates unable to be metabolized in each mutant. Thus, catabolite repression of both enzyme inductions seemed to be under similar regulation. The identification of the possible repressor of the induction of in of inositol dehydrogenase and gluconate kinase in vivo was discussed.
Volume 798(1)
Pages 88-95
Published 1984-3-22
DOI 10.1016/0304-4165(84)90014-x
PII 0304-4165(84)90014-X
PMID 6322857
MeSH Bacillus subtilis / enzymology* Carbohydrates / pharmacology Enzyme Induction Enzyme Repression Genotype Phosphotransferases / biosynthesis* Phosphotransferases (Alcohol Group Acceptor)* Species Specificity Sugar Alcohol Dehydrogenases / biosynthesis*
IF 3.411
Times Cited 50
Prokaryotes B. subtilis MBS69 MBS70 MBS71 MBS72 MBS73 MBS74