Reference - Detail
| RRC ID | 33601 |
|---|---|
| Author | Morimoto T, Ara K, Ozaki K, Ogasawara N. |
| Title | A simple method for introducing marker-free deletions in the Bacillus subtilis genome. |
| Journal | Methods Mol Biol |
| Abstract |
A genetic tool for introducing marker-free deletions is essential for multiple manipulations of genomes. We have developed a simple and efficient method for creating marker-free deletion mutants of Bacillus subtilis through transformation with recombinant PCR products, using the Escherichia coli mazF gene encoding an endoribonuclease that cleaves free mRNAs as a counterselection tool. |
| Volume | 765 |
| Pages | 345-58 |
| Published | 2011-1-1 |
| DOI | 10.1007/978-1-61779-197-0_20 |
| PMID | 21815102 |
| MeSH | Bacillus subtilis / genetics* DNA, Recombinant / genetics* Endoribonucleases / genetics Endoribonucleases / metabolism Escherichia coli / enzymology Escherichia coli / genetics Gene Deletion* Genetic Markers / genetics Genome, Bacterial / genetics* Polymerase Chain Reaction Transformation, Bacterial / genetics* |
| Times Cited | 6 |
| WOS Category | BIOCHEMICAL RESEARCH METHODS GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Prokaryotes B. subtilis | TMO310 TMO311 |