Reference - Detail
| RRC ID | 33602 |
|---|---|
| Author | Morimoto T, Ara K, Ozaki K, Ogasawara N. |
| Title | A new simple method to introduce marker-free deletions in the Bacillus subtilis genome. |
| Journal | Genes Genet Syst |
| Abstract |
A genetic tool to introduce marker-free deletions is essential for multiple manipulations of genomes. We report a simple and efficient method to create marker-free deletion mutants of Bacillus subtilis through transformation with recombinant PCR products, using the Escherichia coli mazF gene encoding an endoribonuclease that cleaves free mRNAs as a counter-selection tool. Our method will be applicable to any bacterium in which introduction of the mazF cassette into the genome by double crossover homologous recombination is possible. |
| Volume | 84(4) |
| Pages | 315-8 |
| Published | 2009-8-1 |
| DOI | 10.1266/ggs.84.315 |
| PII | JST.JSTAGE/ggs/84.315 |
| PMID | 20057169 |
| MeSH | Bacillus subtilis / genetics* DNA-Binding Proteins / genetics Endoribonucleases / genetics Escherichia coli / genetics Escherichia coli Proteins / genetics Gene Deletion* Genetic Markers / genetics Genome, Bacterial / genetics* Recombination, Genetic* |
| IF | 0.917 |
| Times Cited | 29 |
| WOS Category | GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Prokaryotes B. subtilis | TMO310 TMO311 |