| RRC ID |
3364
|
| 著者 |
Nakayama G, Kawaguchi Y, Koga K, Kusakabe T.
|
| タイトル |
Site-specific gene integration in cultured silkworm cells mediated by phiC31 integrase.
|
| ジャーナル |
Mol Genet Genomics
|
| Abstract |
The integrase from the Streptomyces bacteriophage phiC31 carries out efficient recombination between an attP site in the phage genome and an attB site in the host chromosome. In the present study, we have used the phiC31 integrase system to mediate site-specific recombination in the cultured silkworm cell line BmN4. A plasmid containing a cDNA encoding DsRed flanked by two phiC31 attP sites was co-transfected together with a helper plasmid encoding the phiC31 integrase into a cell line in which phiC31 attB sites inserted between a baculovirus IE2 promoter, and a polyadenylation signal are present in one chromosome. Seven days after transfection, expression of DsRed was observed in transformed cells. Nucleotide sequence analysis demonstrated that the expected recombination between the attB and attP sites had been precisely carried out by the phiC31 integrase. These results indicate that the phiC31 site-specific recombination system should be widely applicable for efficient site-specific gene integration into silkworm chromosomes.
|
| 巻・号 |
275(1)
|
| ページ |
1-8
|
| 公開日 |
2006-1-1
|
| DOI |
10.1007/s00438-005-0026-3
|
| PMID |
16341709
|
| MeSH |
Animals
Attachment Sites, Microbiological / genetics*
Bacillus Phages / enzymology*
Baculoviridae / genetics
Bombyx / genetics*
Cell Line
Gene Expression
Gene Targeting*
Integrases / genetics*
Plasmids / genetics
Promoter Regions, Genetic / genetics
RNA 3' Polyadenylation Signals / genetics
Recombination, Genetic
Sequence Analysis, DNA
Streptomyces / virology
Viral Proteins / genetics*
|
| IF |
2.797
|
| 引用数 |
21
|
|
WOS 分野
|
GENETICS & HEREDITY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| カイコ |
|
