RRC ID 3368
著者 Yasukochi Y, Ashakumary LA, Baba K, Yoshido A, Sahara K.
タイトル A second-generation integrated map of the silkworm reveals synteny and conserved gene order between lepidopteran insects.
ジャーナル Genetics
Abstract A second-generation linkage map was constructed for the silkworm, Bombyx mori, focusing on mapping Bombyx sequences appearing in public nucleotide databases and bacterial artificial chromosome (BAC) contigs. A total of 874 BAC contigs containing 5067 clones (22% of the library) were constructed by PCR-based screening with sequence-tagged sites (STSs) derived from whole-genome shotgun (WGS) sequences. A total of 523 BAC contigs, including 342 independent genes registered in public databases and 85 expressed sequence tags (ESTs), were placed onto the linkage map. We found significant synteny and conserved gene order between B. mori and a nymphalid butterfly, Heliconius melpomene, in four linkage groups (LGs), strongly suggesting that using B. mori as a reference for comparative genomics in Lepidotera is highly feasible.
巻・号 173(3)
ページ 1319-28
公開日 2006-7-1
DOI 10.1534/genetics.106.055541
PII genetics.106.055541
PMID 16547103
PMC PMC1526672
MeSH Animals Bombyx / genetics* Chromosome Mapping Chromosomes, Artificial, Bacterial Conserved Sequence Contig Mapping Expressed Sequence Tags Genetic Markers Lepidoptera / cytology Lepidoptera / genetics* Synteny*
IF 4.015
引用数 50
WOS 分野 GENETICS & HEREDITY
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