| RRC ID |
33686
|
| 著者 |
Randlett O, MacDonald RB, Yoshimatsu T, Almeida AD, Suzuki SC, Wong RO, Harris WA.
|
| タイトル |
Cellular requirements for building a retinal neuropil.
|
| ジャーナル |
Cell Rep
|
| Abstract |
How synaptic neuropil is formed within the CNS is poorly understood. The retinal inner plexiform layer (IPL) is positioned between the cell bodies of amacrine cells (ACs) and retinal ganglion cells (RGCs). It consists of bipolar cell (BC) axon terminals that synapse on the dendrites of ACs and RGCs intermingled with projections from Müller glia (MG). We examined whether any of these cellular processes are specifically required for the formation of the IPL. Using genetic and pharmacological strategies, we eliminated RGCs, ACs, and MG individually or in combination. Even in the absence of all of these partner cells, an IPL-like neuropil consisting of only BC axon terminals still forms, complete with presynaptic specializations and sublaminar organization. Previous studies have shown that an IPL can form in the complete absence of BCs; therefore, we conclude that neither presynaptic nor postsynaptic processes are individually essential for the formation of this synaptic neuropil.
|
| 巻・号 |
3(2)
|
| ページ |
282-90
|
| 公開日 |
2013-2-21
|
| DOI |
10.1016/j.celrep.2013.01.020
|
| PII |
S2211-1247(13)00027-2
|
| PMID |
23416047
|
| PMC |
PMC3607253
|
| MeSH |
Amacrine Cells / cytology
Amacrine Cells / pathology
Amacrine Cells / ultrastructure
Animals
Animals, Genetically Modified
Embryo, Nonmammalian / metabolism
Microscopy, Fluorescence
Microscopy, Video
Neurons / pathology
Neuropil / cytology*
Neuropil / pathology
Presynaptic Terminals / pathology
Retina / pathology
Retina / ultrastructure*
Retinal Bipolar Cells / cytology
Retinal Bipolar Cells / pathology
Retinal Bipolar Cells / ultrastructure
Retinal Ganglion Cells / cytology
Retinal Ganglion Cells / pathology
Retinal Ganglion Cells / ultrastructure
Synapses / pathology
Synapses / ultrastructure
Zebrafish / growth & development
|
| IF |
8.109
|
| 引用数 |
23
|
|
WOS 分野
|
CELL BIOLOGY
|
| リソース情報 |
| ゼブラフィッシュ |
Tg(vsx1:GFP) |
