| RRC ID |
3369
|
| Author |
Niimi T, Sahara K, Oshima H, Yasukochi Y, Ikeo K, Traut W.
|
| Title |
Molecular cloning and chromosomal localization of the Bombyx Sex-lethal gene.
|
| Journal |
Genome
|
| Abstract |
We cloned Bm-Sxl, an orthologue of the Drosophila melanogaster Sex-lethal (Sxl) gene from embryos of Bombyx mori. The full-length cDNAs were of 2 sizes, 1528 and 1339 bp, and were named Bm-Sxl-L and Bm-Sxl-S, respectively. Bm-Sxl-L consists of 8 exons and spans more than 20 kb of genomic DNA. The open reading frame (ORF) codes for a protein 336 amino acids in length. Bm-Sxl-S is a splice variant that lacks the second exon. This creates a new translation start 138 nucleotides downstream and an ORF that codes for 46 amino acids fewer at the N-terminus. Linkage analysis using an F2 panel mapped Bm-Sxl to linkage group 16 at 69.8 cM. We isolated 2 BACs that include the Bm-Sxl gene. With BAC-FISH we located Bm-Sxl cytogenetically on the chromosome corresponding to linkage group 16 (LG16) at position >68.8 cM.
|
| Volume |
49(3)
|
| Pages |
263-8
|
| Published |
2006-3-1
|
| DOI |
10.1139/g05-108
|
| PII |
g05-108
|
| PMID |
16604109
|
| MeSH |
Alternative Splicing
Amino Acid Sequence
Animals
Base Sequence
Bombyx / embryology
Bombyx / genetics*
Chromosome Mapping
Chromosomes*
Cloning, Molecular*
DNA / genetics
DNA, Complementary / genetics
Embryo, Nonmammalian
Exons
Genes, Insect*
Genetic Linkage
Genome
In Situ Hybridization, Fluorescence
Insect Proteins / chemistry*
Molecular Sequence Data
Open Reading Frames
Protein Isoforms
|
| IF |
2.037
|
| Times Cited |
32
|
|
WOS Category
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
GENETICS & HEREDITY
|
| Resource |
| Silkworms |
p50
No.459 ? |
