Reference - Detail
| RRC ID | 33707 |
|---|---|
| Author | Katagiri N, Kuroda T, Kishimoto H, Hayashi Y, Kumazawa T, Kimura K. |
| Title | The nucleolar protein nucleophosmin is essential for autophagy induced by inhibiting Pol I transcription. |
| Journal | Sci Rep |
| Abstract |
Various cellular stresses activate autophagy, which is involved in lysosomal degradation of cytoplasmic materials for maintaining nutrient homeostasis and eliminating harmful components. Here, we show that RNA polymerase I (Pol I) transcription inhibition induces nucleolar disruption and autophagy. Treatment with autophagy inhibitors or siRNA specific for autophagy-related (ATG) proteins inhibited autophagy but not nucleolar disruption induced by Pol I transcription inhibition, which suggested that nucleolar disruption was upstream of autophagy. Furthermore, treatment with siRNA specific for nucleolar protein nucleophosmin (NPM) inhibited this type of autophagy. This showed that NPM was involved in autophagy when the nucleolus was disrupted by Pol I inhibition. In contrast, NPM was not required for canonical autophagy induced by nutrient starvation, as it was not accompanied by nucleolar disruption. Thus, our results revealed that, in addition to canonical autophagy, there may be NPM-dependent autophagy associated with nucleolar disruption. |
| Volume | 5 |
| Pages | 8903 |
| Published | 2015-3-10 |
| DOI | 10.1038/srep08903 |
| PII | srep08903 |
| PMID | 25754892 |
| PMC | PMC4354046 |
| MeSH | Autophagy / genetics* Cell Nucleolus / genetics Humans MCF-7 Cells Nuclear Proteins / antagonists & inhibitors Nuclear Proteins / genetics* Nucleolus Organizer Region / genetics Nucleophosmin RNA Polymerase I / antagonists & inhibitors RNA Polymerase I / genetics* RNA, Small Interfering Transcription, Genetic* |
| IF | 3.998 |
| Times Cited | 13 |
| WOS Category | CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | pEGFP-C1-hLC3B (RDB13435) |