RRC ID 33708
Author Kataoka N, Vangnai AS, Pongtharangkul T, Tajima T, Yakushi T, Matsushita K, Kato J.
Title Construction of CoA-dependent 1-butanol synthetic pathway functions under aerobic conditions in Escherichia coli.
Journal J Biotechnol
Abstract 1-Butanol is an important industrial platform chemical and an advanced biofuel. While various groups have attempted to construct synthetic pathways for 1-butanol production, efforts to construct a pathway that functions under aerobic conditions have met with limited success. Here, we constructed a CoA-dependent 1-butanol synthetic pathway that functions under aerobic conditions in Escherichia coli, by expanding the previously reported (R)-1,3-butanediol synthetic pathway. The pathway consists of phaA (acetyltransferase) and phaB (NADPH-dependent acetoacetyl-CoA reductase) from Ralstonia eutropha, phaJ ((R)-specific enoyl-CoA hydratase) from Aeromonas caviae, ter (trans-enoyl-CoA reductase) from Treponema denticola, bld (butylraldehyde dehydrogenase) from Clostridium saccharoperbutylacetonicum, and inherent alcohol dehydrogenase(s) from E. coli. To evaluate the potential of this pathway for 1-butanol production, culture conditions, including volumetric oxygen transfer coefficient (kLa) and pH were optimized in a mini-jar fermenter. Under optimal conditions, 1-butanol was produced at a concentration of up to 8.60gL(-1) after 46h of fed-batch cultivation.
Volume 204
Pages 25-32
Published 2015-6-20
DOI 10.1016/j.jbiotec.2015.03.027
PII S0168-1656(15)00162-5
PMID 25865277
MeSH 1-Butanol / chemistry* Acetyltransferases Aerobiosis Alcohol Dehydrogenase Alcohol Oxidoreductases Aldehyde Dehydrogenase Bacterial Proteins / genetics Bacterial Proteins / metabolism* Bioreactors* Biosynthetic Pathways / genetics Biosynthetic Pathways / physiology* Chromatography, High Pressure Liquid Enoyl-CoA Hydratase Escherichia coli / metabolism* Industrial Microbiology / methods* Plasmids / genetics Spectrophotometry, Ultraviolet
IF 3.503
Times Cited 9
DNA material Genomic DNA of Treponema denticola JCM 8153T (JGD06217)
General Microbes