論文 - 詳細
| RRC ID | 33725 |
|---|---|
| 著者 | Oba M, Demizu Y, Yamashita H, Kurihara M, Tanaka M. |
| タイトル | Plasmid DNA delivery using fluorescein-labeled arginine-rich peptides. |
| ジャーナル | Bioorg Med Chem |
| Abstract |
Arginine (Arg)-rich peptides exhibit an effective cell-penetrating ability and deliver membrane-impermeable compounds into cells. In the present study, three types of Arg-rich peptides, R9 containing nine Arg residues, (RRG)3 containing six Arg and three glycine (Gly) residues, and (RRU)3 containing six Arg and three α-aminoisobutyric acid (Aib) residues, were evaluated for their plasmid DNA (pDNA) delivery and cell-penetrating abilities. The transfection efficiency of R9/pDNA complexes was much higher than those of (RRG)3 and (RRU)3/pDNA complexes, and was derived from the enhanced cellular uptake of R9/pDNA complexes. The replacement of three Arg residues with the neutral amino acid Gly and hydrophobic amino acid Aib drastically changed the cell-penetrating ability and physicochemical properties of peptide/pDNA complexes, resulting in markedly reduced transfection efficiency. A comparison of the R9 peptide administration forms between a peptide alone and peptide/pDNA complex revealed that the uptake of R9 peptides was more efficient for the complex than the peptide alone, but occurred through the same internalization mechanism. The results of the present study will contribute to the design of novel Arg-rich cell-penetrating peptides for pDNA delivery. |
| 巻・号 | 23(15) |
| ページ | 4911-4918 |
| 公開日 | 2015-8-1 |
| DOI | 10.1016/j.bmc.2015.05.025 |
| PII | S0968-0896(15)00425-3 |
| PMID | 26048025 |
| MeSH | Amiloride / pharmacology Amino Acid Sequence Arginine / chemistry* Cell Line, Tumor Cell Survival Endocytosis / drug effects Fluorescein / chemistry* HeLa Cells Humans Microscopy, Confocal Peptides / chemical synthesis Peptides / chemistry* Peptides / metabolism Plasmids / chemistry* Plasmids / metabolism Spectrometry, Fluorescence Temperature Transfection |
| IF | 3.073 |
| 引用数 | 16 |
| WOS 分野 | CHEMISTRY, MEDICINAL CHEMISTRY, ORGANIC BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pCAcc-Luc+ (RDB02443) |