Reference - Detail
| RRC ID | 33953 |
|---|---|
| Author | Dujeancourt L, Richter R, Chrzanowska-Lightowlers ZM, Bonnefoy N, Herbert CJ. |
| Title | Interactions between peptidyl tRNA hydrolase homologs and the ribosomal release factor Mrf1 in S. pombe mitochondria. |
| Journal | Mitochondrion |
| Abstract |
Mitochondrial translation synthesizes key subunits of the respiratory complexes. In Schizosaccharomyces pombe, strains lacking Mrf1, the mitochondrial stop codon recognition factor, are viable, suggesting that other factors can play a role in translation termination. S. pombe contains four predicted peptidyl tRNA hydrolases, two of which (Pth3 and Pth4), have a GGQ motif that is conserved in class I release factors. We show that high dosage of Pth4 can compensate for the absence of Mrf1 and loss of Pth4 exacerbates the lack of Mrf1. Also Pth4 is a component of the mitochondrial ribosome, suggesting that it could help recycling stalled ribosomes. |
| Volume | 13(6) |
| Pages | 871-80 |
| Published | 2013-11-1 |
| DOI | 10.1016/j.mito.2013.07.115 |
| PII | S1567-7249(13)00214-6 |
| PMID | 23892058 |
| PMC | PMC3919214 |
| MeSH | Amino Acid Sequence Carboxylic Ester Hydrolases / metabolism* Gene Deletion Immunoblotting Mitochondria / metabolism* Molecular Sequence Data Schizosaccharomyces / enzymology* Schizosaccharomyces / genetics Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / chemistry Schizosaccharomyces pombe Proteins / metabolism* Sequence Homology, Amino Acid Terminator Regions, Genetic |
| IF | 3.992 |
| Times Cited | 7 |
| WOS Category | GENETICS & HEREDITY CELL BIOLOGY |
| Resource | |
| DNA material | pDUAL-YFH1 (RDB06191) pDUAL-FFH1 (RDB06185) S. pombe YFP-FLAG-His clones SpYFH50B06 (SPW100030) SpYFH41F09 (SPW096529) SpYFH25C12 (SPW090060). |