RRC ID 33965
著者 Schallner N, Pandit R, LeBlanc R 3rd, Thomas AJ, Ogilvy CS, Zuckerbraun BS, Gallo D, Otterbein LE, Hanafy KA.
タイトル Microglia regulate blood clearance in subarachnoid hemorrhage by heme oxygenase-1.
ジャーナル J Clin Invest
Abstract Subarachnoid hemorrhage (SAH) carries a 50% mortality rate. The extravasated erythrocytes that surround the brain contain heme, which, when released from damaged red blood cells, functions as a potent danger molecule that induces sterile tissue injury and organ dysfunction. Free heme is metabolized by heme oxygenase (HO), resulting in the generation of carbon monoxide (CO), a bioactive gas with potent immunomodulatory capabilities. Here, using a murine model of SAH, we demonstrated that expression of the inducible HO isoform (HO-1, encoded by Hmox1) in microglia is necessary to attenuate neuronal cell death, vasospasm, impaired cognitive function, and clearance of cerebral blood burden. Initiation of CO inhalation after SAH rescued the absence of microglial HO-1 and reduced injury by enhancing erythrophagocytosis. Evaluation of correlative human data revealed that patients with SAH have markedly higher HO-1 activity in cerebrospinal fluid (CSF) compared with that in patients with unruptured cerebral aneurysms. Furthermore, cisternal hematoma volume correlated with HO-1 activity and cytokine expression in the CSF of these patients. Collectively, we found that microglial HO-1 and the generation of CO are essential for effective elimination of blood and heme after SAH that otherwise leads to neuronal injury and cognitive dysfunction. Administration of CO may have potential as a therapeutic modality in patients with ruptured cerebral aneurysms.
巻・号 125(7)
ページ 2609-25
公開日 2015-7-1
DOI 10.1172/JCI78443
PII 78443
PMID 26011640
PMC PMC4563677
MeSH Acute-Phase Reaction / cerebrospinal fluid Animals Apoptosis Carbon Monoxide / administration & dosage Carbon Monoxide / metabolism Disease Models, Animal Enzyme Inhibitors / pharmacology Erythrocytes / pathology Female Heme Oxygenase-1 / antagonists & inhibitors Heme Oxygenase-1 / cerebrospinal fluid Heme Oxygenase-1 / deficiency Heme Oxygenase-1 / physiology* Humans Intracranial Aneurysm / cerebrospinal fluid Intracranial Aneurysm / enzymology Male Maze Learning / physiology Membrane Proteins / antagonists & inhibitors Membrane Proteins / deficiency Membrane Proteins / physiology* Metalloporphyrins / pharmacology Mice Mice, Inbred C57BL Mice, Knockout Microglia / enzymology* Microglia / pathology Phagocytosis / physiology Protoporphyrins / pharmacology Subarachnoid Hemorrhage / blood* Subarachnoid Hemorrhage / enzymology* Subarachnoid Hemorrhage / pathology
IF 11.864
引用数 80
WOS 分野 MEDICINE, RESEARCH & EXPERIMENTAL
リソース情報
実験動物マウス RBRC03163