| RRC ID |
34013
|
| Author |
Takahata M, Toh H, Nakano A, Takagi M, Murakami M, Ishii Y, Takizawa T, Tanabe S, Morita H.
|
| Title |
Complete sequence analysis of two cryptic plasmids from Bifidobacterium kashiwanohense JCM 15439 (type strain) isolated from healthy infant feces.
|
| Journal |
Anim Sci J
|
| Abstract |
Bifidobacterial plasmids reported so far are derived from a limited number of strains and plasmids of bifidobacterial type strains isolated from humans are unknown. We found that Bifidobacterium kashiwanohense JCM 15439 (type strain) isolated from a healthy infant contained two cryptic plasmids, designated pBBKW-1 and pBBKW-2. We determined and analyzed the complete sequences of both plasmids. pBBKW-1 (7716 bp) was predicted to replicate by a rolling-circle mechanism and encode six protein-coding genes, two of which are putative replication proteins. pBBKW-1 seems to be a cointegrate plasmid containing two copies of the plasmid pMG1 from Bifidobacterium longum. pBBKW-2 (2920 bp) was predicted to encode six protein-coding genes and be a theta-type replicating plasmid, which has been reported to be more stable than a rolling circle-type replicating plasmid frequently found in bifidobacteria. Our finding will provide new insights into safe recombinant plasmid constructions for humans.
|
| Volume |
85(2)
|
| Pages |
158-63
|
| Published |
2014-2-1
|
| DOI |
10.1111/asj.12095
|
| PMID |
23865717
|
| MeSH |
Base Sequence*
Bifidobacterium / genetics*
Bifidobacterium / isolation & purification
Feces / microbiology*
Humans
Infant
Molecular Sequence Data
Plasmids / genetics*
Sequence Analysis, DNA*
|
| IF |
1.399
|
| Times Cited |
3
|
|
WOS Category
|
AGRICULTURE, DAIRY & ANIMAL SCIENCE
|
| Resource |
| General Microbes |
JCM 15439 |
