RRC ID 34085
著者 Yoshikawa J, Morita T, Fukuoka T, Konishi M, Imura T, Kakugawa K, Kitamoto D.
タイトル Selective production of two diastereomers of disaccharide sugar alcohol, mannosylerythritol by Pseudozyma yeasts.
ジャーナル Appl Microbiol Biotechnol
Abstract Mannosylerythritol (ME) is the hydrophilic backbone of mannosylerythritol lipids as the most promising biosurfactants produced by different Pseudozyma yeasts, and has been receiving attention as a new sugar alcohol. Different Pseudozyma yeasts were examined for the sugar alcohol production using glucose as the sole carbon source. P. hubeiensis KM-59 highly produced a conventional type of ME, i.e., 4-O-β-D-mannopyranosyl-D-erythritol (4-ME). Interestingly, P. tsukubaensis KM-160 produced a diastereomer of 4-ME, i.e., 1-O-β-D-mannopyranosyl-D-erythritol (1-ME). In shake flask culture with 200 g/l of glucose, strain KM-59 produced 4-ME at a yield of 33.2 g/l (2.2 g/l/day of the productivity), while strain KM-160 produced 1-ME at 30.0 g/l (2.0 g/l/day). Moreover, the two strains were found to produce ME from glycerol; the maximum yields of 4-ME and 1-ME from 200 g/l of glycerol were 16.1 g/l (1.1 g/l/day) and 15.8 g/l (1.1 g/l/day), respectively. The production of 1-ME as the new diastereomer was further investigated in fed batch culture using a 5-l jar-fermenter. Compared to the flask culture, strain KM-160 gave three times higher productivity of 1-ME at 38.0 g/l (6.3 g/l/day) from glucose and at 31.1 g/l (3.5 g/l/day) from glycerol, respectively. This is the first report on the selective production of two diastereomers of ME, and should thus facilitate the functional development and application of the disaccharide sugar alcohol in the food and relative industries.
巻・号 98(2)
ページ 823-30
公開日 2014-1-1
DOI 10.1007/s00253-013-5376-x
PMID 24272368
MeSH DNA, Fungal / chemistry DNA, Fungal / genetics DNA, Ribosomal Spacer / chemistry DNA, Ribosomal Spacer / genetics Erythritol / analogs & derivatives* Erythritol / metabolism* Mannosides / metabolism* Molecular Sequence Data Sequence Analysis, DNA Stereoisomerism* Sugar Alcohols / metabolism* Ustilaginales / classification Ustilaginales / genetics Ustilaginales / metabolism*
IF 3.53
引用数 4
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
リソース情報
一般微生物 JCM 3931 JCM 3941 JCM 10317JCM 10318 JCM 10319 JCM 10321 JCM 10323JCM 10324 JCM 11752