RRC ID |
34085
|
著者 |
Yoshikawa J, Morita T, Fukuoka T, Konishi M, Imura T, Kakugawa K, Kitamoto D.
|
タイトル |
Selective production of two diastereomers of disaccharide sugar alcohol, mannosylerythritol by Pseudozyma yeasts.
|
ジャーナル |
Appl Microbiol Biotechnol
|
Abstract |
Mannosylerythritol (ME) is the hydrophilic backbone of mannosylerythritol lipids as the most promising biosurfactants produced by different Pseudozyma yeasts, and has been receiving attention as a new sugar alcohol. Different Pseudozyma yeasts were examined for the sugar alcohol production using glucose as the sole carbon source. P. hubeiensis KM-59 highly produced a conventional type of ME, i.e., 4-O-β-D-mannopyranosyl-D-erythritol (4-ME). Interestingly, P. tsukubaensis KM-160 produced a diastereomer of 4-ME, i.e., 1-O-β-D-mannopyranosyl-D-erythritol (1-ME). In shake flask culture with 200 g/l of glucose, strain KM-59 produced 4-ME at a yield of 33.2 g/l (2.2 g/l/day of the productivity), while strain KM-160 produced 1-ME at 30.0 g/l (2.0 g/l/day). Moreover, the two strains were found to produce ME from glycerol; the maximum yields of 4-ME and 1-ME from 200 g/l of glycerol were 16.1 g/l (1.1 g/l/day) and 15.8 g/l (1.1 g/l/day), respectively. The production of 1-ME as the new diastereomer was further investigated in fed batch culture using a 5-l jar-fermenter. Compared to the flask culture, strain KM-160 gave three times higher productivity of 1-ME at 38.0 g/l (6.3 g/l/day) from glucose and at 31.1 g/l (3.5 g/l/day) from glycerol, respectively. This is the first report on the selective production of two diastereomers of ME, and should thus facilitate the functional development and application of the disaccharide sugar alcohol in the food and relative industries.
|
巻・号 |
98(2)
|
ページ |
823-30
|
公開日 |
2014-1-1
|
DOI |
10.1007/s00253-013-5376-x
|
PMID |
24272368
|
MeSH |
DNA, Fungal / chemistry
DNA, Fungal / genetics
DNA, Ribosomal Spacer / chemistry
DNA, Ribosomal Spacer / genetics
Erythritol / analogs & derivatives*
Erythritol / metabolism*
Mannosides / metabolism*
Molecular Sequence Data
Sequence Analysis, DNA
Stereoisomerism*
Sugar Alcohols / metabolism*
Ustilaginales / classification
Ustilaginales / genetics
Ustilaginales / metabolism*
|
IF |
3.53
|
引用数 |
4
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
リソース情報 |
一般微生物 |
JCM 3931
JCM 3941
JCM 10317JCM 10318
JCM 10319
JCM 10321
JCM 10323JCM 10324
JCM 11752 |