| RRC ID |
34091
|
| 著者 |
Slama N, Mankai H, Ayed A, Mezhoud K, Rauch C, Lazim H, Barkallah I, Gtari M, Limam F.
|
| タイトル |
Streptomyces tunisiensis sp. nov., a novel Streptomyces species with antibacterial activity.
|
| ジャーナル |
Antonie Van Leeuwenhoek
|
| Abstract |
A novel actinomycete strain designated CN-207(T) was isolated from northern Tunisian soil. This strain exhibited potent broad spectrum antibacterial activity against clinical isolates of methicillin-resistant Staphylococcus species and several other Gram-positive and Gram-negative bacteria. Strain CN-207(T) developed greyish aerial mycelium and pale grey substrate mycelium on yeast extract/malt agar. The isolate produced branching vegetative mycelia with sporangiophores bearing sporangia developing at a late stage of growth. The sporangia contained smooth, non-motile spores. Chemotaxonomic characteristics of strain CN-207(T) were typical of the Streptomyces genus. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CN-207(T) belonged to the genus Streptomyces, and was most closely related to Streptomyces griseoincarnatus DSM 40274(T), Streptomyces variabilis DSM 40179(T), Streptomyces labedae DSM 41446(T) and Streptomyces erythrogriseus DSM 40116(T). Low DNA-DNA relatedness values were recorded between strain CN-207(T) and its closest phylogenetic neighbours. Strain CN-207(T) was also distinguished from the nearest phylogenetic neighbours using a combination of morphological and phenotypic characteristics. On the basis of its phenotypic and molecular properties, strain CN-207(T) is considered as a novel species of the Streptomyces genus, for which the name Streptomyces tunisiensis sp. nov. is proposed. The type strain is CN-207(T) (=JCM 17589(T) = DSM 42037(T)).
|
| 巻・号 |
105(2)
|
| ページ |
377-87
|
| 公開日 |
2014-2-1
|
| DOI |
10.1007/s10482-013-0086-z
|
| PMID |
24306769
|
| MeSH |
Antibiosis*
Bacterial Typing Techniques
Cluster Analysis
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
Methicillin-Resistant Staphylococcus aureus / growth & development*
Microscopy, Electron, Scanning
Molecular Sequence Data
Nucleic Acid Hybridization
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Soil Microbiology
Spores, Bacterial / cytology
Streptomyces / classification*
Streptomyces / genetics
Streptomyces / isolation & purification
Streptomyces / physiology*
Tunisia
|
| IF |
2.674
|
| 引用数 |
1
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 17589 |
