Reference - Detail
RRC ID | 34134 |
---|---|
Author | Hayashi H, Kurusu Y. |
Title | DNA regions responsible for maintenance of Shingobium plasmid pYAN-2. |
Journal | Microbes Environ |
Abstract |
We have identified and analyzed two DNA regions responsible for stable maintenance of a plasmid in the genus Sphingomonas and Escherichia coli. A 37 bp fragment, upstream of the repA gene, is required for stable maintenance of the low-copy-number small plasmid pYAN-2 (4,687 bp) from Sphingobium yanoikuyae. It does not encode any significant protein sequence and has one direct repeat for possible secondary structures. Moreover, a 70 bp fragment, upstream of the above sequence, completely stabilized the unstable pSC101 plasmid in E. coli. |
Volume | 29(1) |
Pages | 96-9 |
Published | 2014-1-1 |
DOI | 10.1264/jsme2.me13135 |
PII | DN/JST.JSTAGE/jsme2/ME13135 |
PMID | 24441514 |
PMC | PMC4041236 |
MeSH | Alphaproteobacteria / genetics* Bacterial Proteins / genetics Base Sequence DNA, Bacterial / genetics* Escherichia coli / genetics Gene Dosage Molecular Sequence Data Plasmids / genetics* |
IF | 2.575 |
Times Cited | 1 |
WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
Resource | |
General Microbes | JCM 7371 JCM 7452 JCM 11777 |