論文 - 詳細
| RRC ID | 34158 |
|---|---|
| 著者 | Okahashi N, Sumitomo T, Nakata M, Sakurai A, Kuwata H, Kawabata S. |
| タイトル | Hydrogen peroxide contributes to the epithelial cell death induced by the oral mitis group of streptococci. |
| ジャーナル | PLoS One |
| Abstract |
Members of the mitis group of streptococci are normal inhabitants of the commensal flora of the oral cavity and upper respiratory tract of humans. Some mitis group species, such as Streptococcus oralis and Streptococcus sanguinis, are primary colonizers of the human oral cavity. Recently, we found that hydrogen peroxide (H2O2) produced by S. oralis is cytotoxic to human macrophages, suggesting that streptococcus-derived H2O2 may act as a cytotoxin. Since epithelial cells provide a physical barrier against pathogenic microbes, we investigated their susceptibility to infection by H2O2-producing streptococci in this study. Infection by S. oralis and S. sanguinis was found to stimulate cell death of Detroit 562, Calu-3 and HeLa epithelial cell lines at a multiplicity of infection greater than 100. Catalase, an enzyme that catalyzes the decomposition of H2O2, inhibited S. oralis cytotoxicity, and H2O2 alone was capable of eliciting epithelial cell death. Moreover, S. oralis mutants lacking the spxB gene encoding pyruvate oxidase, which are deficient in H2O2 production, exhibited reduced cytotoxicity toward Detroit 562 epithelial cells. In addition, enzyme-linked immunosorbent assays revealed that both S. oralis and H2O2 induced interleukin-6 production in Detroit 562 epithelial cells. These results suggest that streptococcal H2O2 is cytotoxic to epithelial cells, and promotes bacterial evasion of the host defense systems in the oral cavity and upper respiratory tracts. |
| 巻・号 | 9(1) |
| ページ | e88136 |
| 公開日 | 2014-1-1 |
| DOI | 10.1371/journal.pone.0088136 |
| PII | PONE-D-13-48417 |
| PMID | 24498253 |
| PMC | PMC3909332 |
| MeSH | Bacterial Proteins / metabolism* Cell Death Cell Line Epithelial Cells / metabolism* Epithelial Cells / pathology Humans Hydrogen Peroxide / metabolism* Pyruvate Oxidase / metabolism* Streptococcal Infections / enzymology* Streptococcal Infections / pathology Streptococcus oralis / enzymology* Streptococcus sanguis / enzymology* |
| IF | 2.74 |
| 引用数 | 16 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 12997 |
| ヒト・動物細胞 | HeLa |