| RRC ID |
34190
|
| Author |
Dong L, Ming H, Yin YR, Duan YY, Zhou EM, Nie GX, Feng HG, Liu L, Li WJ.
|
| Title |
Roseomonas alkaliterrae sp. nov., isolated from an alkali geothermal soil sample in Tengchong, Yunnan, South-West China.
|
| Journal |
Antonie Van Leeuwenhoek
|
| Abstract |
An alkalitolerant, thermotolerant and Gram-stain negative bacterium, designated strain YIM 78007(T), was isolated from an alkaline geothermal soil sample from Hehua hot spring, Tengchong, Yunnan province, south-west China. Cells of strain YIM 78007(T) were observed to be aerobic and short rod-shaped. The colonies were observed to be orange-red, convex and circular. 16S rRNA gene sequence-based phylogenetic analysis showed that strain YIM 78007(T) clustered with members of the genus Roseomonas (with similarities from 97.2 to 92.2 %). Optimal growth of strain YIM 78007 occurs at 40-50 °C and pH 8.0-10.0. The predominant ubiquinone was identified as Q-10 and the major fatty acids were identified as C18:1 ω7c and C16:0. The polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, two unidentified aminolipids and one unknown phospholipid. The G + C content of the genomic DNA was determined to be 63 mol %. The levels of DNA-DNA hybridization relatedness between strain YIM 78007(T) and its closet neighbours (Roseomonas lacus JCM 13283(T) and Roseomonas terrae JCM 14592(T)) were well below the threshold required for the proposal of a novel species. The results of physiological and biochemical characteristics, the phylogenetic analysis, as well as low DNA-DNA hybridization values, allowed the phenotypic and genotypic differentiation of strain YIM 78007(T) from its closest phylogenetic neighbours. Therefore, strain YIM 78007(T) is considered to represent a novel species of the genus Roseomonas, for which the name Roseomonas alkaliterrae sp. nov. is proposed. The type strain is YIM 78007(T) (=BCRC 80644(T) = JCM 19656(T)).
|
| Volume |
105(5)
|
| Pages |
899-905
|
| Published |
2014-5-1
|
| DOI |
10.1007/s10482-014-0144-1
|
| PMID |
24590559
|
| MeSH |
Bacterial Typing Techniques
Base Composition
China
Cluster Analysis
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
Fatty Acids / analysis
Hot Springs
Methylobacteriaceae / classification*
Methylobacteriaceae / genetics
Methylobacteriaceae / isolation & purification*
Methylobacteriaceae / physiology
Molecular Sequence Data
Nucleic Acid Hybridization
Phospholipids / analysis
Phylogeny
Quinones / analysis
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Soil Microbiology*
|
| IF |
2.674
|
| Times Cited |
15
|
|
WOS Category
|
MICROBIOLOGY
|
| Resource |
| General Microbes |
JCM 13283
JCM 14592
JCM 19656 |
