| RRC ID |
34202
|
| 著者 |
Cha IT, Yim KJ, Song HS, Lee HW, Hyun DW, Kim KN, Choi JS, Kim D, Lee SJ, Seo MJ, Choi HJ, Bae JW, Rhee SK, Roh SW, Nam YD.
|
| タイトル |
Halolamina rubra sp. nov., a haloarchaeon isolated from non-purified solar salt.
|
| ジャーナル |
Antonie Van Leeuwenhoek
|
| Abstract |
Two Gram-stain negative, rod-shaped and motile extreme halophiles, designated CBA1107(T) and CBA1108, were isolated from non-purified solar salt. Based on the phylogenetic analysis, strains CBA1107(T) and CBA1108 were shown to belong to the genus Halolamina, with similarities for the 16S rRNA gene sequences between strains CBA1107(T) and Halolamina pelagica TBN21(T) , Halolamina salina WSY15-H3(T) and Halolamina salifodinae WSY15-H1(T) of 98.3, 97.6 and 97.3 %, respectively; the similarities for the rpoB' gene sequences between the same strains were 96.0, 95.3 and 94.6 %, respectively. The colonies of both strains were observed to be red pigmented on growth medium. Strain CBA1107(T) was observed to grow at 20-50 °C, in the presence of 15-30 % NaCl, at pH 6.0-9.0, and with 0.005-0.5 M Mg(2+). The cells of both strains lysed in distilled water. The DNA-DNA hybridization experiments showed that strain CBA1107(T) shared 97 % relatedness with CBA1108 and <50 % relatedness with H. pelagica JCM 16809(T), H. salina JCM 18549(T) and H. salifodinae JCM 18548(T). The genomic DNA G+C content of strain CBA1107(T) was determined to be 65.1 mol%. The major polar lipids of the two strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and glycolipids including sulfated mannosyl glucosyl diether and mannosyl glucosyl diether. Based on the polyphasic taxonomic analyses, the strains are considered to represent a new taxon for which the name Halolamina rubra sp. nov. is proposed, with the type strain CBA1107(T) (=CECT 8421(T) =JCM 19436(T)).
|
| 巻・号 |
105(5)
|
| ページ |
907-14
|
| 公開日 |
2014-5-1
|
| DOI |
10.1007/s10482-014-0145-0
|
| PMID |
24633912
|
| MeSH |
Base Composition
Cluster Analysis
DNA, Archaeal / chemistry
DNA, Archaeal / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
Environmental Microbiology*
Glycolipids / analysis
Halobacteriaceae / classification*
Halobacteriaceae / genetics
Halobacteriaceae / isolation & purification*
Halobacteriaceae / physiology
Molecular Sequence Data
Nucleic Acid Hybridization
Phospholipids / analysis
Phylogeny
RNA, Ribosomal, 16S / genetics
Salts / metabolism*
Sequence Analysis, DNA
|
| IF |
2.674
|
| 引用数 |
9
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 16809
JCM 18548
JCM 18549
JCM 19436
JCM 19437 |
