| RRC ID |
34209
|
| Author |
Takei S, Omoto C, Kitagawa K, Morishita N, Katayama T, Shigemura K, Fujisawa M, Kawabata M, Hotta H, Shirakawa T.
|
| Title |
Oral administration of genetically modified Bifidobacterium displaying HCV-NS3 multi-epitope fusion protein could induce an HCV-NS3-specific systemic immune response in mice.
|
| Journal |
Vaccine
|
| Abstract |
More than 170 million people worldwide are chronic HCV (Hepatitis C virus) carriers, and about 30% of them will develop progressive liver disease, such as cirrhosis and hepatocellular carcinoma. A combination of pegylated interferon-α with ribavirin, the standard treatment for HCV infection, has been effective in fewer than 50% of patients infected with HCV genotype 1. A strong T cell response against the nonstructural protein 3 (NS3) is important for recovery from acute HCV infection, and an early multi-specific CD4+ helper and CD8+ cytotoxic T cell response is critical for HCV clearance. In the present study, we successfully constructed a genetically modified Bifidobacterium longum (B. longum) displaying recombinant HCV-NS3 peptides containing some CD4 and CD8 epitopes located in the HCV-NS3 region as an oral vaccine against chronic HCV infection. The oral administration of this vaccine could induce NS3-specific immune responses in mice through intestinal mucosal immunity. Our findings suggest that this novel oral vaccine has great potential as a novel oral vaccine against chronic HCV infection.
|
| Volume |
32(25)
|
| Pages |
3066-74
|
| Published |
2014-5-23
|
| DOI |
10.1016/j.vaccine.2014.03.022
|
| PII |
S0264-410X(14)00366-1
|
| PMID |
24657718
|
| MeSH |
Administration, Oral
Animals
Antibodies, Viral / immunology
Bifidobacterium* / genetics
Bifidobacterium* / immunology
Cytokines / immunology
Epitopes / immunology*
Female
Hepatitis C / prevention & control*
Immunity, Mucosal
Intestines / immunology
Mice, Inbred BALB C
Recombinant Fusion Proteins / immunology
Spleen / immunology
T-Lymphocytes / immunology
Viral Hepatitis Vaccines / immunology*
Viral Nonstructural Proteins / immunology*
|
| IF |
3.143
|
| Times Cited |
16
|
|
WOS Category
|
MEDICINE, RESEARCH & EXPERIMENTAL
IMMUNOLOGY
|
| Resource |
| General Microbes |
JCM 1217 |
