RRC ID 34277
Author Egan M, O'Connell Motherway M, Ventura M, van Sinderen D.
Title Metabolism of sialic acid by Bifidobacterium breve UCC2003.
Journal Appl Environ Microbiol
Abstract Bifidobacteria constitute a specific group of commensal bacteria that inhabit the gastrointestinal tracts of humans and other mammals. Bifidobacterium breve UCC2003 has previously been shown to utilize several plant-derived carbohydrates that include cellodextrins, starch, and galactan. In the present study, we investigated the ability of this strain to utilize the mucin- and human milk oligosaccharide (HMO)-derived carbohydrate sialic acid. Using a combination of transcriptomic and functional genomic approaches, we identified a gene cluster dedicated to the uptake and metabolism of sialic acid. Furthermore, we demonstrate that B. breve UCC2003 can cross feed on sialic acid derived from the metabolism of 3'-sialyllactose, an abundant HMO, by another infant gut bifidobacterial strain, Bifidobacterium bifidum PRL2010.
Volume 80(14)
Pages 4414-26
Published 2014-7-1
DOI 10.1128/AEM.01114-14
PII AEM.01114-14
PMID 24814790
PMC PMC4068672
MeSH Bifidobacterium / genetics Bifidobacterium / metabolism* Culture Media DNA Fragmentation DNA, Bacterial / genetics Multigene Family N-Acetylneuraminic Acid / metabolism* Oligonucleotide Array Sequence Analysis Plasmids / genetics Sequence Analysis, DNA
IF 4.016
Times Cited 69
WOS Category BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
Resource
General Microbes JCM 7017 JCM 7019