論文 - 詳細
| RRC ID | 34296 |
|---|---|
| 著者 | Mora M, Bellack A, Ugele M, Hopf J, Wirth R. |
| タイトル | The temperature gradient-forming device, an accessory unit for normal light microscopes to study the biology of hyperthermophilic microorganisms. |
| ジャーナル | Appl Environ Microbiol |
| Abstract |
To date, the behavior of hyperthermophilic microorganisms in their biotope has been studied only to a limited degree; this is especially true for motility. One reason for this lack of knowledge is the requirement for high-temperature microscopy-combined, in most cases, with the need for observations under strictly anaerobic conditions-for such studies. We have developed a custom-made, low-budget device that, for the first time, allows analyses in temperature gradients up to 40°C over a distance of just 2 cm (a biotope-relevant distance) with heating rates up to ∼5°C/s. Our temperature gradient-forming device can convert any upright light microscope into one that works at temperatures as high as 110°C. Data obtained by use of this apparatus show how very well hyperthermophiles are adapted to their biotope: they can react within seconds to elevated temperatures by starting motility-even after 9 months of storage in the cold. Using the temperature gradient-forming device, we determined the temperature ranges for swimming, and the swimming speeds, of 15 selected species of the genus Thermococcus within a few months, related these findings to the presence of cell surface appendages, and obtained the first evidence for thermotaxis in Archaea. |
| 巻・号 | 80(15) |
| ページ | 4764-70 |
| 公開日 | 2014-8-1 |
| DOI | 10.1128/AEM.00984-14 |
| PII | AEM.00984-14 |
| PMID | 24858087 |
| PMC | PMC4148812 |
| MeSH | Equipment Design Hot Temperature Microscopy / instrumentation Microscopy / methods* Thermococcus / chemistry Thermococcus / classification Thermococcus / cytology* |
| IF | 4.016 |
| 引用数 | 6 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 12380 |