RRC ID 34299
著者 Jin Y, Kim YJ, Hoang VA, Young Jung S, Nguyen NL, Woo Min J, Wang C, Yang DC.
タイトル Flavobacterium panaciterrae sp. nov., a β-glucosidase producing bacterium with ginsenoside-converting activity isolated from the soil of a ginseng field.
ジャーナル J Gen Appl Microbiol
Abstract The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain DCY69(T) is JX233806. A Gram-reaction-negative, oxidase- and catalase-positive, non-gliding motile strain, designated strain DCY69(T), was isolated from the soil of a ginseng field in the Republic of Korea. Colonies of strain DCY69(T) were circular, 0.5-1.5 mm diameter, yellow, and convex on an R2A agar plate after 2 days. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain DCY69(T) belonged to the genus Flavobacterium with 90.5-98.3% gene sequence similarity. The major predominant quinone was MK-6. The major cellular fatty acids were iso-C15:0, iso-C17:0 3-OH, iso-C15:0 3-OH and summed feature 3 (containing C16:1ω7c and/or C16:1ω6c). The major polar lipids were phosphatidylethanolamine, one unidentified aminolipid and unidentified polar lipids (L1, L2). The genomic DNA G+C content of strain DCY69(T) was 35.0mol%. The strain DCY69(T) transformed ginsenoside Rb1 into Rd and F2. Based on the polyphasic taxonomic data, strain DCY69(T) is considered to represent a novel species of the genus Flavobacterium, for which the name Flavobacterium panaciterrae sp. nov. is proposed. The type strain is DCY69(T)(= KCTC 32392(T) = JCM 19161(T)), isolated from the soil of a ginseng field in the Republic of Korea.
巻・号 60(2)
ページ 59-64
公開日 2014-1-1
DOI 10.2323/jgam.60.59
PII DN/JST.JSTAGE/jgam/60.59
PMID 24859863
MeSH Bacterial Typing Techniques Base Composition Cluster Analysis Cytosol / chemistry DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Fatty Acids / analysis Flavobacterium / classification* Flavobacterium / genetics Flavobacterium / isolation & purification* Flavobacterium / metabolism Ginsenosides / metabolism* Glucosidases / metabolism* Locomotion Molecular Sequence Data Panax / growth & development Phospholipids / analysis Phylogeny Pigments, Biological / metabolism Quinones / analysis RNA, Ribosomal, 16S / genetics Republic of Korea Sequence Analysis, DNA Soil Microbiology*
IF 1.442
引用数 5
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
リソース情報
一般微生物 JCM 19161