論文 - 詳細
| RRC ID | 34345 |
|---|---|
| 著者 | Kasahara K, Ishikawa H, Sato S, Shimakawa Y, Watanabe K. |
| タイトル | Development of multiplex loop-mediated isothermal amplification assays to detect medically important yeasts in dairy products. |
| ジャーナル | FEMS Microbiol Lett |
| Abstract |
Rapid detection of yeast contamination is important in the food industry. We have developed loop-mediated isothermal amplification (LAMP) assays to detect the emerging opportunistic pathogenic yeasts: Candida albicans, Candida glabrata, Candida tropicalis, the Candida parapsilosis group, Trichosporon asahii, and Trichosporon mucoides. These yeasts may cause deep-seated candidiasis or trichosporonosis. Four LAMP primer sets specific for Candida were designed to target the internal transcribed spacer 2 (ITS2) region between the 5.8S and 26S rRNA genes, and two LAMP primer sets specific for Trichosporon were designed to target the intergenic spacer 1 (IGS1) region between the 26S and 5S rRNA genes. The LAMP assays could detect these yeasts in a range between 10(0) and 10(3) cells mL(-1) in a contaminated dairy product within 1 h. We also developed multiplex LAMP assays to detect these Candida or Trichosporon species in a single reaction. Multiplex LAMP assays can detect contamination if at least one of the target species is present; they are more time- and cost-efficient than conventional methods and could detect target yeasts with sensitivity close to that of the LAMP assays. Multiplex LAMP assays established in this study can be used as a primary screening method for yeast contamination in food products. |
| 巻・号 | 357(2) |
| ページ | 208-16 |
| 公開日 | 2014-8-1 |
| DOI | 10.1111/1574-6968.12512 |
| PMID | 24965944 |
| MeSH | Candida / classification* Candida / genetics Candida / isolation & purification* DNA Primers / genetics DNA, Fungal / genetics DNA, Ribosomal Spacer / genetics Dairy Products / microbiology* Microbiological Techniques / methods* Nucleic Acid Amplification Techniques / methods* Sensitivity and Specificity Time Factors Trichosporon / classification* Trichosporon / genetics Trichosporon / isolation & purification* |
| IF | 1.987 |
| 引用数 | 9 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 1810 JCM 1811 |