| RRC ID |
34357
|
| Author |
Chen J, Bowman KS, Rainey FA, Moe WM.
|
| Title |
Reassessment of PCR primers targeting 16S rRNA genes of the organohalide-respiring genus Dehalogenimonas.
|
| Journal |
Biodegradation
|
| Abstract |
Representatives from the genus Dehalogenimonas have the metabolic capacity to anaerobically transform a variety of environmentally important polychlorinated aliphatic compounds. In light of the recent isolation of additional strains, description of a new species, and an expanded number of uncultured DNA sequences, PCR primers and protocols intended to uniquely target members of this organohalide-respiring genus were reevaluated. Nine of fourteen primer combinations reported previously as genus-specific failed to amplify 16S rRNA genes of recently isolated Dehalogenimonas strains. Use of alternative combinations or modified genus-specific primers, however, allowed detection of all presently known Dehalogenimonas strains. Use of a modified primer set in qPCR revealed an approximately two-order of magnitude increase in concentration of Dehalogenimonas 16S rRNA gene copies following subsurface injection of electron donors at a Louisiana Superfund site, demonstrating the utility of the newly developed protocol and suggesting that the genus Dehalogenimonas can respond to biostimulation remediation strategies in a manner similar to that previously reported for other dechlorinating genera such as Dehalococcoides.
|
| Volume |
25(5)
|
| Pages |
747-56
|
| Published |
2014-9-1
|
| DOI |
10.1007/s10532-014-9696-z
|
| PMID |
24989478
|
| MeSH |
Biodegradation, Environmental
Chloroflexi / metabolism*
DNA Primers / genetics
Polymerase Chain Reaction
RNA, Ribosomal, 16S / genetics*
|
| IF |
2.805
|
| Times Cited |
8
|
|
WOS Category
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| Resource |
| General Microbes |
JCM 17062
JCM 19277 |
