RRC ID 34414
著者 Jung HJ, Cha IT, Yim KJ, Song HS, Cho K, Kim D, Lee HW, Lee JK, Seo MJ, Roh SW, Lee SJ.
タイトル Citrimicrobium luteum gen. nov., sp. nov., aerobic anoxygenic phototrophic bacterium isolated from the gut of a sea cucumber Stichopus japonicus.
ジャーナル J Microbiol
Abstract A Gram-stain negative, yellow-pigmented, motile, pleomorphic bacterium, designated strain CBA4602(T), was isolated from the gut of the sea cucumber Stichopus japonicus, which was collected from Jeju Island in the Republic of Korea. In a phylogenetic analysis based on the 16S rRNA gene, strain CBA4602(T) belonged to the order Sphingomonadales in the class Alphaproteobacteria. The 16S rRNA gene sequence similarity between strain CBA4602(T) and 'Citromicrobium bathyomarinum' JF-1, the most closely related strain having nonvalidly published name, was 98.4%, followed by 95.2-96.7% identities with sequence of the other closest strains in the genus Erythrobacter. Strain CBA4602(T) had bacteriochlorophyll a and carotenoids. Strain CBA4602(T) grew in 0-10% (w/v) NaCl, at 10-42°C and pH 6.0-8.0, with optimal growth in 1-2% NaCl, at 30-37°C and pH 7.0. Strain CBA4602(T) was positive for catalase and oxidase activities and was able to hydrolyse gelatine and Tween 20 and 40, but not starch, Tween 80 or L-tyrosine. The G+C content of genomic DNA from strain CBA4602(T) was 68.0 mol% and Q-10 was the major detected isoprenoid quinone. The polar lipids were three unidentified phospholipids, three unidentified glycolipids, and two unidentified lipids. The dominant fatty acids were anteiso-C15:0, C16:0, anteiso-C17:0 and C18:0. As considering the current taxonomic status of the genus 'Citromicrobium' and polyphasic taxonomic analyses, strain CBA4602(T) represents a novel genus and species. The name Citrimicrobium luteum is proposed for the type strain CBA4602(T) (=KACC 17668(T) =JCM 19530(T)).
巻・号 52(10)
ページ 819-24
公開日 2014-10-1
DOI 10.1007/s12275-014-4136-7
PMID 25163838
MeSH Aerobiosis Alphaproteobacteria / classification* Alphaproteobacteria / genetics Alphaproteobacteria / isolation & purification* Alphaproteobacteria / physiology Animals Base Composition Cluster Analysis Cytosol / chemistry DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Enzymes / analysis Fatty Acids / analysis Gastrointestinal Tract / microbiology Glycolipids / analysis Locomotion Molecular Sequence Data Phospholipids / analysis Phylogeny Pigments, Biological / metabolism Quinones / analysis RNA, Ribosomal, 16S / genetics Republic of Korea Sequence Analysis, DNA Sodium Chloride / metabolism Stichopus / microbiology* Temperature
IF 2.845
引用数 0
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 19530