| RRC ID |
34448
|
| 著者 |
Li QQ, Zhou XK, Dang LZ, Cheng J, Hozzein WN, Liu MJ, Hu Q, Li WJ, Duan YQ.
|
| タイトル |
Paenibacillus nicotianae sp. nov., isolated from a tobacco sample.
|
| ジャーナル |
Antonie Van Leeuwenhoek
|
| Abstract |
A Gram-stain positive, facultative anaerobic endospore-forming bacterium, designated strain YIM h-19(T), was isolated from a tobacco sample. Cells were observed to be motile rods by means of peritrichous flagella and colonies were observed to be convex, yellow, circular and showed catalase-positive and oxidase-negative reactions. Strain YIM h-19(T) was able to grow at 4-45 °C, pH 6.0-8.0 and 0-3 % NaCl (w/v). The predominant respiratory quinone was identified as MK-7. Major fatty acids were identified as anteiso-C15:0, anteiso-C17:0 and C16:0. The polar lipids were found to be phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and two unidentified polar lipids. The genomic DNA G+C content was determined to be 54 mol%. 16S rRNA gene sequence analysis showed the strain YIM h-19(T) was most closely related to Paenibacillus hordei RH-N24(T) and Paenibacillus hunanensis FeL05(T) with similarities of 98.30 and 94.64 % respectively. However, DNA-DNA hybridization data indicated that the isolate represented a novel genomic species with the genus Paenibacillus. All data from genotypic and phenotypic analyses support the conclusion that strain YIM h-19(T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus nicotianae sp. nov. is proposed. The type strain is YIM h-19(T) (=CGMCC1.12819(T) = NRRL B-59112(T)).
|
| 巻・号 |
106(6)
|
| ページ |
1199-205
|
| 公開日 |
2014-12-1
|
| DOI |
10.1007/s10482-014-0289-y
|
| PMID |
25239270
|
| MeSH |
Bacterial Typing Techniques
Base Composition
Cluster Analysis
Cytosol / chemistry
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
Fatty Acids / analysis
Hydrogen-Ion Concentration
Locomotion
Molecular Sequence Data
Nucleic Acid Hybridization
Paenibacillus / classification*
Paenibacillus / genetics
Paenibacillus / isolation & purification*
Paenibacillus / physiology
Phospholipids / analysis
Phylogeny
Quinones / analysis
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Sodium Chloride / metabolism
Temperature
Tobacco / microbiology*
|
| IF |
2.674
|
| 引用数 |
4
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 17570 |
