Reference - Detail
| RRC ID | 34463 |
|---|---|
| Author | Ujihara T, Nagano M, Wada H, Mitsuhashi S. |
| Title | Identification of a novel type of polyunsaturated fatty acid synthase involved in arachidonic acid biosynthesis. |
| Journal | FEBS Lett |
| Abstract |
Arachidonic acid (ARA) is a polyunsaturated fatty acid (PUFA) and an essential component of membrane lipids. However, the PUFA synthase required for ARA biosynthesis has not been identified in any organism. To identify the PUFA synthase producing ARA, we determined the draft genome sequence of the marine bacterium Aureispira marina, which produces a high level of ARA, and found a gene cluster encoding a putative PUFA synthase for ARA production. Expression of the gene cluster in Escherichia coli induced production of ARA, demonstrating that the gene cluster encodes a PUFA synthase required for ARA biosynthesis. |
| Volume | 588(21) |
| Pages | 4032-6 |
| Published | 2014-11-3 |
| DOI | 10.1016/j.febslet.2014.09.023 |
| PII | S0014-5793(14)00700-5 |
| PMID | 25263707 |
| MeSH | Arachidonic Acid / biosynthesis* Bacteroidetes / enzymology Bacteroidetes / genetics Bacteroidetes / metabolism Escherichia coli / genetics Fatty Acid Synthases / genetics* Fatty Acid Synthases / metabolism* Multigene Family |
| IF | 3.057 |
| Times Cited | 9 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Resource | |
| General Microbes | JCM 23201 |