RRC ID 3448
Author Zhao AC, Zhao TF, Nakagaki K, Zhang YS, Sima YH, Miao YG, Shiomi K, Kajiura Z, Nagata Y, Takadera M, Nakagaki M.
Title Novel molecular and mechanical properties of egg case silk from wasp spider, Argiope bruennichi.
Journal Biochemistry
Abstract Araneoid spiders use specialized abdominal glands to produce up to seven different protein-based silks/glues that have various mechanical properties. To date, the fibroin sequences encoding egg case fibers have not been fully determined. To gain further understanding of a recently reported spider silk protein gene family, several novel strategies were utilized in this study to isolate two full-length cDNAs of egg case silk proteins, cylindrical silk protein 1 (CySp1, 9.1 kb) and cylindrical silk protein 2 (CySp2, 9.8 kb), from the wasp spider, Argiope bruennichi. Northern blotting analysis demonstrated that CySp1 and CySp2 are selectively expressed in the cylindrical glands. The amino acid composition of raw egg case silk was closely consistent with the deduced amino acid composition based on the sequences of CySp1 and CySp2, which supports the assertion that CySp1 and CySp2 represent two major components of egg case silk. CySp1 and CySp2 are primarily composed of remarkable homogeneous assemble repeats that are 180 residues in length and consist of several complex subrepeats, and they contain highly homologous C-termini and markedly different N-termini. Our results suggest a possible link between CySp1 and CySp2. In addition, comparisons of stress/strain curves for dragline and egg case silk from Argiope bruennichi showed obvious differences in ultimate strength and extensibility, and similarities in toughness.
Volume 45(10)
Pages 3348-56
Published 2006-3-14
DOI 10.1021/bi052414g
PMID 16519529
MeSH Amino Acid Sequence Animals Cloning, Molecular DNA, Complementary / genetics DNA, Complementary / isolation & purification Female Insect Proteins / genetics Insect Proteins / metabolism* Molecular Sequence Data Ovum / metabolism Sequence Alignment Sequence Analysis, Protein Sequence Homology, Amino Acid Silk / chemistry Silk / genetics Silk / metabolism Spiders / genetics Spiders / metabolism* Structure-Activity Relationship
IF 2.997
Times Cited 35
Silkworms ?