論文 - 詳細
| RRC ID | 34493 |
|---|---|
| 著者 | Tanaka Y, Benno Y. |
| タイトル | Application of a single-colony coculture technique to the isolation of hitherto unculturable gut bacteria. |
| ジャーナル | Microbiol Immunol |
| Abstract |
Molecular studies have led to postulation of a relationship between gut microbiota and certain diseases. However, because studies of hitherto uncultured species in vivo are essential for characterizing the biology and pathogenic properties of gut bacteria, techniques for culturing and isolating such bacteria must be developed. Here, a technique is described that partially overcomes the obstacles that prevent detection of interbacterial communication in vitro and are thus responsible for the failure to culture certain bacterial species. For this purpose, a ring with a membrane filter at the bottom was designed and a relatively simple nutrient medium was used instead of conventional media. Gut bacteria were cocultivated in soft agar separated by the membrane filter to simulate interbacterial communication in vitro. Use of this soft agar coculture technique led to the successful isolation of hitherto uncultured bacteria and the demonstration of multistage interbacterial communication among gut bacteria in vitro. Cultivation and isolation of single colonies of bacteria that require other bacteria for growth will enhance efforts to better understand the physiological and pathogenic roles of gut microbiota. |
| 巻・号 | 59(2) |
| ページ | 63-70 |
| 公開日 | 2015-2-1 |
| DOI | 10.1111/1348-0421.12220 |
| PMID | 25510436 |
| MeSH | Adult Bacteria / growth & development* Bacteria / isolation & purification* Bacteriological Techniques / methods* Gastrointestinal Tract / microbiology* Humans Microbial Interactions |
| IF | 1.566 |
| 引用数 | 11 |
| WOS 分野 | MICROBIOLOGY IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 5826 JCM 11019 JCM 13223 JCM 13471 |