論文 - 詳細
| RRC ID | 34515 |
|---|---|
| 著者 | Skovgaard S, Larsen MH, Nielsen LN, Skov RL, Wong C, Westh H, Ingmer H. |
| タイトル | Recently introduced qacA/B genes in Staphylococcus epidermidis do not increase chlorhexidine MIC/MBC. |
| ジャーナル | J Antimicrob Chemother |
| Abstract |
OBJECTIVES:Chlorhexidine is used as a disinfectant to prevent surgical infections. Recently, studies have indicated that chlorhexidine usage has selected methicillin-resistant Staphylococcus aureus strains that are tolerant to chlorhexidine and that this may be related to the presence of the qacA/B-encoded efflux pumps. Here, we evaluated if high-level exposure to chlorhexidine selects for tolerant colonizing Staphylococcus epidermidis and we addressed the consequences of long-term exposure to chlorhexidine. METHODS:Chlorhexidine susceptibility and carriage of qacA/B was determined for colonizing S. epidermidis isolated from scrub nurses heavily exposed to chlorhexidine and were compared with isolates from non-users of chlorhexidine hand rubs. S. epidermidis blood isolates from the 1960s, before the wider introduction of chlorhexidine to the market, were also tested and compared with recently collected S. epidermidis blood isolates. RESULTS:There was no correlation between the use of chlorhexidine in scrub nurses and the presence of qacA/B genes in S. epidermidis isolates or increased MICs/MBCs of chlorhexidine for S. epidermidis isolates. While 55% of current blood isolates harboured the qacA/B genes, none of the 33 historical S. epidermidis isolates did, although their MICs and MBCs of chlorhexidine were comparable to those for current isolates. CONCLUSIONS:Chlorhexidine used as a hand rub does not select for S. epidermidis isolates with increased MICs or MBCs of chlorhexidine. However, the absence of qacA/B genes in S. epidermidis isolates obtained in the 1960s suggests that long-term use of biocides like chlorhexidine or related compounds may select for the presence of qacA/B genes. |
| 巻・号 | 68(10) |
| ページ | 2226-33 |
| 公開日 | 2013-10-1 |
| DOI | 10.1093/jac/dkt182 |
| PII | dkt182 |
| PMID | 23739538 |
| MeSH | Bacterial Proteins / genetics* Chlorhexidine / pharmacology* Disinfectants / pharmacology* Drug Tolerance* Gene Transfer, Horizontal* Hand Disinfection / methods Humans Infection Control / methods Membrane Transport Proteins / genetics* Microbial Sensitivity Tests Nurses Staphylococcus epidermidis / drug effects* Staphylococcus epidermidis / genetics Staphylococcus epidermidis / isolation & purification |
| IF | 5.439 |
| 引用数 | 18 |
| WOS 分野 | PHARMACOLOGY & PHARMACY INFECTIOUS DISEASES MICROBIOLOGY |
| リソース情報 | |
| 一般微生物 | JCM 16555 |