RRC ID 34515
Author Skovgaard S, Larsen MH, Nielsen LN, Skov RL, Wong C, Westh H, Ingmer H.
Title Recently introduced qacA/B genes in Staphylococcus epidermidis do not increase chlorhexidine MIC/MBC.
Journal J. Antimicrob. Chemother.
Abstract OBJECTIVES:Chlorhexidine is used as a disinfectant to prevent surgical infections. Recently, studies have indicated that chlorhexidine usage has selected methicillin-resistant Staphylococcus aureus strains that are tolerant to chlorhexidine and that this may be related to the presence of the qacA/B-encoded efflux pumps. Here, we evaluated if high-level exposure to chlorhexidine selects for tolerant colonizing Staphylococcus epidermidis and we addressed the consequences of long-term exposure to chlorhexidine.
METHODS:Chlorhexidine susceptibility and carriage of qacA/B was determined for colonizing S. epidermidis isolated from scrub nurses heavily exposed to chlorhexidine and were compared with isolates from non-users of chlorhexidine hand rubs. S. epidermidis blood isolates from the 1960s, before the wider introduction of chlorhexidine to the market, were also tested and compared with recently collected S. epidermidis blood isolates.
RESULTS:There was no correlation between the use of chlorhexidine in scrub nurses and the presence of qacA/B genes in S. epidermidis isolates or increased MICs/MBCs of chlorhexidine for S. epidermidis isolates. While 55% of current blood isolates harboured the qacA/B genes, none of the 33 historical S. epidermidis isolates did, although their MICs and MBCs of chlorhexidine were comparable to those for current isolates.
CONCLUSIONS:Chlorhexidine used as a hand rub does not select for S. epidermidis isolates with increased MICs or MBCs of chlorhexidine. However, the absence of qacA/B genes in S. epidermidis isolates obtained in the 1960s suggests that long-term use of biocides like chlorhexidine or related compounds may select for the presence of qacA/B genes.
Volume 68(10)
Pages 2226-33
Published 2013-10
DOI 10.1093/jac/dkt182
PII dkt182
PMID 23739538
MeSH Bacterial Proteins / genetics* Chlorhexidine / pharmacology* Disinfectants / pharmacology* Drug Tolerance* Gene Transfer, Horizontal* Hand Disinfection / methods Humans Infection Control / methods Membrane Transport Proteins / genetics* Microbial Sensitivity Tests Nurses Staphylococcus epidermidis / drug effects* Staphylococcus epidermidis / genetics Staphylococcus epidermidis / isolation & purification
IF 5.217
Times Cited 8
WOS Category PHARMACOLOGY & PHARMACY INFECTIOUS DISEASES MICROBIOLOGY
Resource
General Microbes JCM 16555