RRC ID 34563
著者 Eto S, Miyamoto H, Shobuike T, Noda I, Akiyama T, Tsukamoto M, Ueno M, Someya S, Kawano S, Sonohata M, Mawatari M.
タイトル Silver oxide-containing hydroxyapatite coating supports osteoblast function and enhances implant anchorage strength in rat femur.
ジャーナル J Orthop Res
Abstract Antibacterial silver with hydroxyapatite (Ag-HA) is a promising coating material for imparting antibacterial properties to implants. We previously reported that 3% (w/w) silver with HA (3% Ag-HA) has both antibacterial activity and osteoconductivity. In this study, we investigated the effects of Ag-HA on the in vitro osteoblast function and the in vivo anchorage strength and osteoconductivity of implants. Production of the osteoblast marker alkaline phosphatase, but not cytotoxicity, was observed in cells of the osteoblast cell line MC3T3-E1 cultured on the 3% Ag-HA-coated surface. These results were similar to those observed with silver-free HA coating. In contrast, a significant high level of cytotoxicity was observed when the cells were cultured on a 50% Ag-HA-coated surface. The anchorage strength of implants inserted into the femur of Sprague-Dawley (SD) rats was enhanced by coating the implants with 3% Ag-HA. On the 3% Ag-HA-coated surface, both metaphyseal and diaphyseal areas were largely covered with new bone and had adequate osteoconductivity. These results suggest that 3% Ag-HA, like conventional HA, promotes osteogenesis by supporting osteoblast viability and function and thereby contributes to sufficient anchorage strength of implants. Application of 3% Ag-HA, which combines the osteoconductivity of HA and the antibacterial activity of silver, to prosthetic joints will help prevent postoperative infections.
巻・号 33(9)
ページ 1391-7
公開日 2015-9-1
DOI 10.1002/jor.22903
PMID 25808232
MeSH Alkaline Phosphatase / chemistry Animals Anti-Bacterial Agents / pharmacology Bone and Bones / pathology Cell Line Cell Proliferation Coated Materials, Biocompatible / chemistry* Durapatite / chemistry* Femur / pathology* Joint Prosthesis* Materials Testing Osteoblasts / cytology Osteoblasts / drug effects* Osteogenesis / drug effects Oxides / chemistry* Rats Rats, Sprague-Dawley Silver Compounds / chemistry* Staphylococcus aureus / drug effects Titanium / chemistry
IF 3.414
引用数 10
リソース情報
ヒト・動物細胞 MC3T3-E1(RCB1126)