RRC ID 34648
Author Shen XF, Chu FF, Lam PK, Zeng RJ.
Title Biosynthesis of high yield fatty acids from Chlorella vulgaris NIES-227 under nitrogen starvation stress during heterotrophic cultivation.
Journal Water Res
Abstract In this study the heterotrophic cultivation of Chlorella vulgaris NIES-227 fed with glucose was investigated systematically using six media types; combinations of nitrogen repletion/depletion and phosphorus repletion/limitation/depletion. It was found that a high yield of fatty acids (0.88 of fed glucose-COD) and a high content of fatty acid methyl esters (FAMEs) (89% of dry weight) were obtained under nitrogen starved conditions. To our knowledge it is the first report on such high COD conversion yield and FAME content in microalgae. The dominant fatty acid (>50%) was methyl oleate (C18:1), a desirable component for biodiesel synthesis. FAME content under nitrogen starved conditions was significantly higher than under nitrogen sufficient conditions, while phosphorus had no significant influence, indicating that nitrogen starvation was the real "fatty acids trigger" in heterotrophic cultivation. These findings could simplify the downstream extraction process, such as the extrusion of oil from soybeans, and could reduce operating costs by improving the fatty acid yield from waste COD.
Volume 81
Pages 294-300
Published 2015-9-15
DOI 10.1016/j.watres.2015.06.003
PII S0043-1354(15)30051-8
PMID 26081436
MeSH Biofuels / microbiology Chlorella vulgaris / growth & development Chlorella vulgaris / metabolism* Fatty Acids / biosynthesis* Glucose / metabolism Heterotrophic Processes Lipids / biosynthesis Nitrogen / deficiency* Phosphorus / metabolism
IF 9.13
Times Cited 41
Algae NIES-227