| RRC ID |
3465
|
| Author |
Mauchamp B, Royer C, Garel A, Jalabert A, Da Rocha M, Grenier AM, Labas V, Vinh J, Mita K, Kadono K, Chavancy G.
|
| Title |
Polycalin (chlorophyllid A binding protein): a novel, very large fluorescent lipocalin from the midgut of the domestic silkworm Bombyx mori L.
|
| Journal |
Insect Biochem Mol Biol
|
| Abstract |
We studied a protein from the midgut of the silkworm Bombyx mori characterized by its ability to bind the prosthetic group of chlorophyll, that confers fluorescent properties to this protein. Several techniques, 2D electrophoresis purification, MS-MS and Maldi-TOF peptide sequencing, RT-PCR and nucleotide sequencing were used to obtain the nucleotide sequence and the deduced amino acid sequence. The coding sequence was compared to the gene sequence to define the number and size of introns and exons. The gene spanned 45.5 kb of DNA and consisted of 46 exons. The cDNA encoded a protein of 2721 amino acids. The protein was identified as a lipocalin with novel features. Most lipocalins are proteins with high affinity to small lipophilic molecules, with a molecular size in the 25 kDa range and a well conserved tertiary structure. The apoprotein described here revealed 15 lipocalin like structures, in line. We called this protein a polycalin (pentadecacalin).
|
| Volume |
36(8)
|
| Pages |
623-33
|
| Published |
2006-8-1
|
| DOI |
10.1016/j.ibmb.2006.05.006
|
| PII |
S0965-1748(06)00112-3
|
| PMID |
16876705
|
| MeSH |
Amino Acid Sequence
Animals
Bombyx / chemistry*
Bombyx / genetics
Gastrointestinal Tract / chemistry
Genome, Insect
Insect Proteins / analysis
Insect Proteins / chemistry*
Insect Proteins / genetics
Molecular Sequence Data
Sequence Analysis, DNA
Spectrometry, Mass, Electrospray Ionization
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
|
| IF |
3.827
|
| Times Cited |
25
|
|
WOS Category
|
ENTOMOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Silkworms |
|
