RRC ID 3468
Author Katsuma S, Horie S, Daimon T, Iwanaga M, Shimada T.
Title In vivo and in vitro analyses of a Bombyx mori nucleopolyhedrovirus mutant lacking functional vfgf.
Journal Virology
Abstract All lepidopteran baculovirus genomes sequenced to date encode a viral fibroblast growth factor homolog (vfgf), suggesting that vfgf may play an important role in the infection cycle of lepidopteran baculoviruses. Here, we describe the characterization of a Bombyx mori nucleopolyhedrovirus (BmNPV) mutant lacking functional vfgf. We constructed a vfgf deletion mutant (BmFGFD) and characterized it in BmN cells and B. mori larvae. We observed that budded virus (BV) production was reduced in BmFGFD-infected BmN cells and B. mori larvae. The larval bioassays also revealed that deletion of vfgf did not reduce the infectivity; however, the mutant virus did take 20 h longer to kill B. mori larvae than wild-type BmNPV, when tested either by BV injection or by polyhedrin-inclusion body ingestion. These results suggest that BmNPV vfgf is involved in efficient virus production in BmN cells and B. mori larvae.
Volume 355(1)
Pages 62-70
Published 2006-11-10
DOI 10.1016/j.virol.2006.07.008
PII S0042-6822(06)00447-8
PMID 16904150
MeSH Animals Bombyx / virology* Cell Line DNA, Viral / biosynthesis Electrophoresis, Polyacrylamide Gel Gene Deletion Gene Expression Genes, Viral Growth Substances / genetics Growth Substances / physiology* Larva / virology Nucleopolyhedrovirus / genetics Nucleopolyhedrovirus / pathogenicity* Nucleopolyhedrovirus / physiology Polymerase Chain Reaction RNA, Messenger / biosynthesis RNA, Viral / biosynthesis Reverse Transcriptase Polymerase Chain Reaction Viral Plaque Assay Viral Proteins / biosynthesis Viral Proteins / genetics Viral Proteins / physiology* Virulence / genetics Virus Assembly Virus Replication
IF 3.374
Times Cited 35
Silkworms ?