| RRC ID |
3468
|
| 著者 |
Katsuma S, Horie S, Daimon T, Iwanaga M, Shimada T.
|
| タイトル |
In vivo and in vitro analyses of a Bombyx mori nucleopolyhedrovirus mutant lacking functional vfgf.
|
| ジャーナル |
Virology
|
| Abstract |
All lepidopteran baculovirus genomes sequenced to date encode a viral fibroblast growth factor homolog (vfgf), suggesting that vfgf may play an important role in the infection cycle of lepidopteran baculoviruses. Here, we describe the characterization of a Bombyx mori nucleopolyhedrovirus (BmNPV) mutant lacking functional vfgf. We constructed a vfgf deletion mutant (BmFGFD) and characterized it in BmN cells and B. mori larvae. We observed that budded virus (BV) production was reduced in BmFGFD-infected BmN cells and B. mori larvae. The larval bioassays also revealed that deletion of vfgf did not reduce the infectivity; however, the mutant virus did take 20 h longer to kill B. mori larvae than wild-type BmNPV, when tested either by BV injection or by polyhedrin-inclusion body ingestion. These results suggest that BmNPV vfgf is involved in efficient virus production in BmN cells and B. mori larvae.
|
| 巻・号 |
355(1)
|
| ページ |
62-70
|
| 公開日 |
2006-11-10
|
| DOI |
10.1016/j.virol.2006.07.008
|
| PII |
S0042-6822(06)00447-8
|
| PMID |
16904150
|
| MeSH |
Animals
Bombyx / virology*
Cell Line
DNA, Viral / biosynthesis
Electrophoresis, Polyacrylamide Gel
Gene Deletion
Gene Expression
Genes, Viral
Growth Substances / genetics
Growth Substances / physiology*
Larva / virology
Nucleopolyhedroviruses / genetics
Nucleopolyhedroviruses / pathogenicity*
Nucleopolyhedroviruses / physiology
Polymerase Chain Reaction
RNA, Messenger / biosynthesis
RNA, Viral / biosynthesis
Reverse Transcriptase Polymerase Chain Reaction
Viral Plaque Assay
Viral Proteins / biosynthesis
Viral Proteins / genetics
Viral Proteins / physiology*
Virulence / genetics
Virus Assembly
Virus Replication
|
| IF |
2.819
|
| 引用数 |
40
|
|
WOS 分野
|
VIROLOGY
|
| リソース情報 |
| カイコ |
|
