RRC ID 34757
著者 Nozaki S, Yamada Y, Ogawa T.
タイトル Initiator titration complex formed at datA with the aid of IHF regulates replication timing in Escherichia coli.
ジャーナル Genes Cells
Abstract The initiation of replication in Escherichia coli is negatively controlled by a mechanism referred to as 'initiator titration', a process by which the initiator protein, DnaA, is titrated to newly replicated binding sequences on the chromosome to reduce the initiation potential for replication. Initiator titration occurs predominantly at the datA locus that binds exceptionally large amounts of DnaA molecules to prevent aberrant initiations. We found that this was enabled by integration host factor (IHF). Within datA, there is a consensus IHF recognition sequence between the two DnaA recognition sequences (DnaA boxes) essential for its function. Binding of IHF to this site was demonstrated both in vitro and in vivo. Disruption of the core sequence in the consensus of the IHF-binding resulted in increased origin concentration as observed in Delta datA cells. Furthermore, the number of DnaA molecules bound to datA was reduced in cells carrying a disruption in the IHF-binding core sequence. The IHF-binding site and the essential DnaA boxes had to be located at a proper distance and orientation to maintain the accurate initiation timing. Therefore, IHF is a unique element in the control of replication initiation that acts negatively at datA, while known to act as a positive regulator at oriC.
巻・号 14(3)
ページ 329-41
公開日 2009-3-1
DOI 10.1111/j.1365-2443.2008.01269.x
PII GTC1269
PMID 19170757
MeSH Bacterial Proteins / metabolism Base Sequence DNA Replication* DNA-Binding Proteins / metabolism Escherichia coli / metabolism* Integration Host Factors / metabolism* Molecular Sequence Data Replication Origin Surface Plasmon Resonance
IF 1.655
引用数 25
WOS 分野 GENETICS & HEREDITY CELL BIOLOGY
リソース情報
原核生物(大腸菌) ME9746 ME9747 ME9748 ME9749 ME9750 ME9751 ME9752 ME9753 ME9754 ME9755 ME9756 ME9757 ME9758 ME9759 ME9760 ME9761 ME9762 ME9763 ME9764 ME9765 ME9766 ME9767 ME9768