RRC ID 34760
著者 Kitani T, Yoda K, Ogawa T, Okazaki T.
タイトル Evidence that discontinuous DNA replication in Escherichia coli is primed by approximately 10 to 12 residues of RNA starting with a purine.
ジャーナル J Mol Biol
Abstract Intact primer RNA for discontinuous DNA replication of Escherichia coli has been detected by specific labeling in vitro of its 5'-terminal tri- (or di-) phosphate group with vaccinia guanylyltransferase and [alpha-32P]GTP. A mutant defective either in RNase H or in both RNase H and DNA polymerase I accumulated about 10 or 30 times more intact primer RNA, respectively, than wild-type cells. The primers started with purine in an A to G ratio of 5 and the most abundant 5'-terminal dinucleotide sequence was (p)ppA-Pu. The chain length of the intact primer RNA was approximately 10 to 12 nucleotide residues. The structural properties of the E. coli primer RNa resemble those of the eukaryotic primer RNA.
巻・号 184(1)
ページ 45-52
公開日 1985-7-5
DOI 10.1016/0022-2836(85)90042-7
PII 0022-2836(85)90042-7
PMID 2411935
MeSH Base Sequence DNA Replication* DNA, Bacterial / genetics* Electrophoresis, Polyacrylamide Gel Escherichia coli / genetics Purines RNA / genetics* RNA, Bacterial / genetics*
IF 4.76
引用数 77
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(大腸菌) ME9700